HitPredict

Interactor 1: P85B (O00459)
Phosphatidylinositol 3-kinase regulatory subunit beta (PI3-kinase regulatory subunit beta) (PI3K regulatory subunit beta) (PtdIns-3-kinase regulatory subunit beta) (Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta) (PI3-kinase subunit p85-beta) (PtdIns-3-kinase regulatory subunit p85-beta)
Gene: PIK3R2

Interactor 2: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.76	1.00	0.875

Property	Details
Experiments	19380743, 20936779, 21706016
Identification Methods	affinity chromatography technology, two hybrid, tandem affinity purification, two hybrid pooling approach, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	PI3K_P85_iSH2~SH2, SH2~SH3_1
Common GO terms	GO:0001784 - phosphotyrosine residue binding GO:0005634 - nucleus GO:0005829 - cytosol GO:0008286 - insulin receptor signaling pathway GO:0019903 - protein phosphatase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 728 Hs			1 217
+	Hs
		129163 P85B_HUMAN (1-728): 0: 549: 100.00		LCK_HUMAN (3-202): 3.69e-37: 137: 28.91
+	Hs
		129151 P85B_HUMAN (1-728): 0: 549: 100.00		ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46
+	Hs
		69198 P85B_HUMAN (1-728): 0: 549: 100.00		A9UF07_HUMAN (4-139): 1.63e-21: 94: 35.46
+	Hs
		129164 P85B_HUMAN (1-728): 0: 549: 100.00		FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58
+	Hs
		129173 P85B_HUMAN (1-728): 0: 549: 100.00		HCK_HUMAN (120-215): 1.21e-12: 68.1: 19.39
+	Hs
		129169 P85B_HUMAN (1-728): 0: 549: 100.00		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		129185 P85B_HUMAN (1-728): 0: 549: 100.00		SRC_HUMAN (161-215): 3.76e-11: 63.8: 31.58
+	Hs
		924735 P55G_HUMAN (312-722): 1.53e-103: 326: 71.85		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Hs
		557757 P55G_HUMAN (312-722): 1.53e-103: 326: 71.85		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Hs
		789082 P55G_HUMAN (312-722): 1.53e-103: 326: 71.85		ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04
+	Hs
		412285 P55G_HUMAN (312-722): 1.53e-103: 326: 71.85		FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58
+	Hs
		433433 P55G_HUMAN (312-722): 1.53e-103: 326: 71.85		YES_HUMAN (59-215): 4.17e-13: 69.2: 14.91
+	Hs
		442864 P55G_HUMAN (312-722): 1.53e-103: 326: 71.85		HCK_HUMAN (120-215): 1.21e-12: 68.1: 19.39
+	Hs
		433926 P55G_HUMAN (312-722): 1.53e-103: 326: 71.85		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		523089 P55G_HUMAN (312-722): 1.53e-103: 326: 71.85		SRC_HUMAN (161-215): 3.76e-11: 63.8: 31.58
+	Hs
		789020 TXK_HUMAN (550-727): 8.04e-33: 135: 23.24		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Dm
		337676 SRC64_DROME (550-722): 2.59e-33: 137: 18.23		GRAP_DROME (1-215): 5.1e-62: 194: 62.79
+	Dm
		337641 ABL_DROME (531-725): 1.27e-32: 138: 20.92		ABL_DROME (4-135): 1e-22: 97.4: 37.68
+	Dm
		1360389 SRC42_DROME (551-727): 1.47e-35: 143: 22.78		SRC42_DROME (131-215): 1.35e-11: 65: 19.54
+	Ce
		220205 SRC2_CAEEL (550-726): 8.48e-34: 138: 21.11		SEM5_CAEEL (3-69): 6.24e-14: 70: 28.36