HitPredict

Interactor 1: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Interactor 2: UB2E2 (Q96LR5)
Ubiquitin-conjugating enzyme E2 E2 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme E2) (UbcH8) (Ubiquitin carrier protein E2) (Ubiquitin-protein ligase E2)
Gene: UBE2E2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.73	1.00	0.858

Property	Details
Experiments	33762435, 30017913, 19549727
Identification Methods	affinity chromatography technology, pull down, two hybrid
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	HECT~UQ_con
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0070534 - protein K63-linked ubiquitination GO:0070936 - protein K48-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 903 Hs			1 201
+	Hs
		1282329 ITCH_HUMAN (1-903): 0: 926: 100.00		UB2E3_HUMAN (1-201): 6.37e-85: 251: 79.71
+	Hs
		1294888 ITCH_HUMAN (1-903): 0: 926: 100.00		UB2D4_HUMAN (55-200): 9.37e-83: 243: 63.01
+	Hs
		919827 ITCH_HUMAN (1-903): 0: 926: 100.00		UB2D2_HUMAN (55-200): 2.8e-82: 242: 64.38
+	Hs
		849411 ITCH_HUMAN (1-903): 0: 926: 100.00		UB2D1_HUMAN (55-200): 1.23e-81: 240: 61.64
+	Hs
		851871 ITCH_HUMAN (1-903): 0: 926: 100.00		UB2E1_HUMAN (28-201): 6.5e-80: 238: 89.71
+	Hs
		895882 ITCH_HUMAN (1-903): 0: 926: 100.00		UB2D3_HUMAN (55-200): 1.28e-80: 238: 63.70
+	Hs
		125655 WWP2_HUMAN (1-903): 0: 816: 54.08		UB2E2_HUMAN (1-201): 6.02e-94: 274: 100.00
+	Hs
		919860 WWP1_HUMAN (1-903): 0: 832: 57.54		UB2D2_HUMAN (55-200): 2.8e-82: 242: 64.38
+	Hs
		849444 WWP1_HUMAN (1-903): 0: 832: 57.54		UB2D1_HUMAN (55-200): 1.23e-81: 240: 61.64
+	Hs
		895910 WWP1_HUMAN (1-903): 0: 832: 57.54		UB2D3_HUMAN (55-200): 1.28e-80: 238: 63.70
+	Hs
		125629 WWP2_HUMAN (1-903): 0: 816: 54.08		UB2E3_HUMAN (1-201): 6.37e-85: 251: 79.71
+	Hs
		125808 WWP2_HUMAN (1-903): 0: 816: 54.08		UB2D4_HUMAN (55-200): 9.37e-83: 243: 63.01
+	Hs
		125335 WWP2_HUMAN (1-903): 0: 816: 54.08		UB2D2_HUMAN (55-200): 2.8e-82: 242: 64.38
+	Hs
		125262 WWP2_HUMAN (1-903): 0: 816: 54.08		UB2D1_HUMAN (55-200): 1.23e-81: 240: 61.64
+	Hs
		125263 WWP2_HUMAN (1-903): 0: 816: 54.08		UB2E1_HUMAN (28-201): 6.5e-80: 238: 89.71
+	Hs
		125300 WWP2_HUMAN (1-903): 0: 816: 54.08		UB2D3_HUMAN (55-200): 1.28e-80: 238: 63.70
+	Dm
		854579 SUDX_DROME (19-903): 0: 788: 48.29		UBCD2_DROME (40-201): 7.11e-78: 234: 87.65
+	Xl
		1151480 Q6GMD5_XENLA (3-900): 0: 765: 31.40		Q6WKX0_XENLA (1-201): 1.51e-84: 250: 79.71
+	Hs
		849416 NED4L_HUMAN (5-900): 0: 762: 32.14		UB2D1_HUMAN (55-200): 1.23e-81: 240: 61.64
+	Hs
		851875 NED4L_HUMAN (5-900): 0: 762: 32.14		UB2E1_HUMAN (28-201): 6.5e-80: 238: 89.71