HitPredict

Interactor 1: NEDD8 (Q9VJ33)
Ubiquitin-like protein NEDD8 (Neddylin)
Gene: NEDD8

Interactor 2: UBC2M (Q9VSF3)
Nedd8-conjugating enzyme UbcE2M (EC 2.3.2.34) (Nedd8 carrier protein)
Gene: UbcE2M

Species: Drosophila melanogaster

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.34	1.00	0.590

Property	Details
Experiments	19079254
Identification Methods	two hybrid array
Interaction Type	physical association

Property	Details
Interacting Pfam domains	UQ_con~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0045116 - protein neddylation GO:0045879 - negative regulation of smoothened signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 84 Dm			1 181
+	Hs
		982194 NEDD8_HUMAN (1-76): 1.23e-39: 127: 93.42		UB2D2_HUMAN (26-170): 7.58e-77: 227: 31.29
+	Hs
		982162 RS27A_HUMAN (1-77): 3.6e-37: 123: 57.14		UB2D2_HUMAN (26-170): 7.58e-77: 227: 31.29
+	Hs
		957057 RS27A_HUMAN (1-77): 3.6e-37: 123: 57.14		UB2D3_HUMAN (26-170): 1.7e-75: 224: 30.61
+	Hs
		73732 B2RDW1_HUMAN (1-77): 3.6e-37: 123: 57.14		UB2E3_HUMAN (9-170): 1.21e-73: 222: 29.88
+	Hs
		982163 RL40_HUMAN (1-81): 3.28e-35: 117: 54.32		UB2D2_HUMAN (26-170): 7.58e-77: 227: 31.29
+	Hs
		507431 UBB_HUMAN (1-76): 5.66e-32: 112: 57.90		UB2D4_HUMAN (26-170): 1.89e-77: 229: 30.61
+	Hs
		957058 RL40_HUMAN (1-81): 3.28e-35: 117: 54.32		UB2D3_HUMAN (26-170): 1.7e-75: 224: 30.61
+	Hs
		507147 UBB_HUMAN (1-76): 5.66e-32: 112: 57.90		UB2D2_HUMAN (26-170): 7.58e-77: 227: 31.29
+	Hs
		507136 UBB_HUMAN (1-76): 5.66e-32: 112: 57.90		UB2D3_HUMAN (26-170): 1.7e-75: 224: 30.61
+	At
		762746 UBQ3_ARATH (1-77): 1.01e-29: 108: 58.44		UBC8_ARATH (26-171): 4.11e-77: 228: 32.43
+	Hs
		1217033 Q5U5U6_HUMAN (1-76): 5.66e-32: 112: 57.90		UB2E3_HUMAN (9-170): 1.21e-73: 222: 29.88
+	Hs
		507301 UBB_HUMAN (1-76): 5.66e-32: 112: 57.90		UB2E3_HUMAN (9-170): 1.21e-73: 222: 29.88
+	At
		762762 UBQ3_ARATH (1-77): 1.01e-29: 108: 58.44		UBC9_ARATH (26-171): 3.01e-76: 226: 31.08
+	At
		762777 UBQ3_ARATH (1-77): 1.01e-29: 108: 58.44		UBC10_ARATH (26-171): 3.25e-76: 226: 31.76
+	At
		762787 UBQ3_ARATH (1-77): 1.01e-29: 108: 58.44		UBC11_ARATH (26-171): 2.24e-76: 226: 33.11
+	Hs
		957399 NEDD8_HUMAN (1-76): 1.23e-39: 127: 93.42		UBC12_HUMAN (1-179): 2.65e-68: 207: 73.63
+	Hs
		957306 RS27A_HUMAN (1-77): 3.6e-37: 123: 57.14		UBC12_HUMAN (1-179): 2.65e-68: 207: 73.63
+	Hs
		507116 UBB_HUMAN (1-76): 5.66e-32: 112: 57.90		UBE2A_HUMAN (24-169): 2.67e-69: 208: 29.73
+	At
		683037 UBQ3_ARATH (1-77): 1.01e-29: 108: 58.44		UBC1_ARATH (24-172): 6.66e-70: 210: 27.15
+	At
		842109 UBQ3_ARATH (1-77): 1.01e-29: 108: 58.44		UBC2_ARATH (24-172): 9.15e-70: 210: 27.15