HitPredict

Interactor 1: SH21B (O14796)
SH2 domain-containing protein 1B (EWS/FLI1-activated transcript 2) (EAT-2)
Gene: SH2D1B

Interactor 2: ACK1 (Q07912)
Activated CDC42 kinase 1 (ACK-1) (EC 2.7.10.2) (EC 2.7.11.1) (Tyrosine kinase non-receptor protein 2)
Gene: TNK2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, validated two hybrid, two hybrid array, two hybrid prey pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PK_Tyr_Ser-Thr~SH2
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 132 Hs			1 1038
+	Hs
		176332 SHIP2_HUMAN (5-102): 1.32e-36: 133: 42.86		ABL1_HUMAN (1-955): 4.84e-144: 462: 19.03
+	Hs
		337307 FYN_HUMAN (3-125): 5.83e-35: 128: 22.48		ABL1_HUMAN (1-955): 4.84e-144: 462: 19.03
+	Hs
		69199 SHIP2_HUMAN (5-102): 1.32e-36: 133: 42.86		A9UF07_HUMAN (1-955): 2.36e-134: 447: 19.03
+	Hs
		155837 SH21B_HUMAN (1-132): 1.12e-39: 131: 100.00		FAK1_HUMAN (6-692): 3.3e-131: 425: 21.01
+	Hs
		176447 SHIP2_HUMAN (5-102): 1.32e-36: 133: 42.86		ERBB4_HUMAN (84-754): 3.34e-128: 423: 24.17
+	Hs
		155844 SH21B_HUMAN (1-132): 1.12e-39: 131: 100.00		ERBB4_HUMAN (84-754): 3.34e-128: 423: 24.17
+	Mm
		710126 FYN_MOUSE (3-125): 5.55e-35: 128: 22.48		FAK1_MOUSE (6-692): 1.85e-131: 426: 21.28
+	Mm
		763386 FYN_MOUSE (3-125): 5.55e-35: 128: 22.48		EPHA4_MOUSE (84-486): 2.21e-125: 408: 24.22
+	Mm
		763392 SH21C_MOUSE (1-132): 1.09e-41: 136: 65.91		FYN_MOUSE (1-400): 4.06e-126: 396: 30.18
+	Hs
		155829 SH21B_HUMAN (1-132): 1.12e-39: 131: 100.00		SRC_HUMAN (1-400): 3.99e-127: 399: 31.42
+	Dm
		1360389 SRC42_DROME (3-131): 3.55e-36: 131: 26.52		SRC42_DROME (1-400): 4.94e-126: 395: 29.46
+	Hs
		411936 FYN_HUMAN (3-125): 5.83e-35: 128: 22.48		FYN_HUMAN (1-400): 2.52e-126: 397: 30.18
+	Mm
		536205 FGR_MOUSE (3-124): 4.13e-35: 128: 24.22		FGR_MOUSE (1-401): 1.3e-125: 394: 30.60
+	Hs
		411545 FYN_HUMAN (3-125): 5.83e-35: 128: 22.48		LCK_HUMAN (1-398): 8.72e-125: 391: 30.08
+	Mm
		639445 SH21C_MOUSE (1-132): 1.09e-41: 136: 65.91		LYN_MOUSE (92-388): 4.67e-112: 358: 37.46
+	Hs
		176351 SHIP2_HUMAN (5-102): 1.32e-36: 133: 42.86		LYN_HUMAN (92-388): 4.52e-112: 358: 36.79
+	Hs
		176333 SHIP2_HUMAN (5-102): 1.32e-36: 133: 42.86		EGFR_HUMAN (105-387): 2.98e-55: 211: 39.58
+	Hs
		155822 SH21B_HUMAN (1-132): 1.12e-39: 131: 100.00		EGFR_HUMAN (105-387): 2.98e-55: 211: 39.58
+	Mm
		435883 SH21C_MOUSE (1-132): 1.09e-41: 136: 65.91		HCK_MOUSE (108-385): 1.38e-52: 195: 37.99
+	Hs
		238330 FYN_HUMAN (3-125): 5.83e-35: 128: 22.48		JAK2_HUMAN (102-413): 4.21e-17: 89: 26.97