HitPredict

Interactor 1: NFYB3 (O23310)
Nuclear transcription factor Y subunit B-3 (AtNF-YB-3) (AtNF-YB3) (Transcriptional activator HAP3C)
Gene: NFYB3

Interactor 2: NFYC9 (Q8L4B2)
Nuclear transcription factor Y subunit C-9 (AtNF-YC-9) (Transcriptional activator HAP5C)
Gene: NFYC9

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.80	1.00	0.898

Property	Details
Experiments	25105952, 22199235, 22912760, 28650476
Identification Methods	two hybrid, protein complementation assay, Cr-two hybrid, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	CBFD_NFYB_HMF~Histone
Common GO terms	GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0003700 - DNA-binding transcription factor activity GO:0005634 - nucleus GO:0046982 - protein heterodimerization activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 161 At			1 231
+	At
		188289 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		NFYC3_ARATH (1-231): 5.89e-84: 250: 80.09
+	At
		1196497 NFYB6_ARATH (6-145): 5.91e-56: 177: 52.45		NFYC9_ARATH (1-231): 1.07e-92: 273: 100.00
+	At
		188281 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		NFYC4_ARATH (21-228): 1.78e-71: 220: 54.42
+	At
		188287 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		NFYC1_ARATH (41-228): 2.63e-71: 219: 58.03
+	At
		188269 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		NFYC8_ARATH (44-229): 2.46e-60: 189: 38.54
+	At
		188270 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		NFYCA_ARATH (18-206): 1.26e-59: 188: 28.57
+	At
		188273 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		NFYC2_ARATH (59-209): 1.17e-57: 183: 66.23
+	At
		188278 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		NFYC5_ARATH (49-222): 2.31e-55: 177: 38.20
+	At
		188279 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		NFYC6_ARATH (50-228): 3.1e-54: 174: 43.41
+	At
		188280 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		NFYC7_ARATH (51-229): 2.13e-51: 167: 33.86
+	At
		1117859 NFYB6_ARATH (6-145): 5.91e-56: 177: 52.45		NFYC8_ARATH (44-229): 2.46e-60: 189: 38.54
+	At
		1123155 NFYB6_ARATH (6-145): 5.91e-56: 177: 52.45		NFYCA_ARATH (18-206): 1.26e-59: 188: 28.57
+	At
		1196498 NFYB6_ARATH (6-145): 5.91e-56: 177: 52.45		NFYC2_ARATH (59-209): 1.17e-57: 183: 66.23
+	At
		1196500 NFYB6_ARATH (6-145): 5.91e-56: 177: 52.45		NFYC5_ARATH (49-222): 2.31e-55: 177: 38.20
+	At
		188282 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		Q9LHG0_ARATH (73-191): 7.66e-34: 124: 26.89
+	At
		188283 NFYB3_ARATH (1-161): 3.68e-74: 220: 100.00		DPB31_ARATH (64-177): 3.74e-33: 120: 21.05
+	Hs
		627431 NFYB_HUMAN (3-155): 1.65e-57: 180: 52.26		NFYC_HUMAN (56-229): 4.96e-45: 157: 45.46
+	Rn
		927731 NFYB_RAT (3-155): 1.22e-56: 177: 51.61		NFYC_RAT (56-229): 2.43e-46: 158: 45.46
+	Hs
		627432 NFYB_HUMAN (3-155): 1.65e-57: 180: 52.26		NC2A_HUMAN (70-227): 2.17e-38: 133: 21.55
+	Hs
		627441 NFYB_HUMAN (3-155): 1.65e-57: 180: 52.26		DPOE4_HUMAN (72-153): 6.03e-27: 101: 35.37