HitPredict

Interactor 1: PJA2 (O43164)
E3 ubiquitin-protein ligase Praja-2 (Praja2) (EC 2.3.2.27) (RING finger protein 131) (RING-type E3 ubiquitin transferase Praja-2)
Gene: PJA2

Interactor 2: UB2D2 (P62837)
Ubiquitin-conjugating enzyme E2 D2 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D2) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D2) (Ubiquitin carrier protein D2) (Ubiquitin-conjugating enzyme E2(17)KB 2) (Ubiquitin-conjugating enzyme E2-17 kDa 2) (Ubiquitin-protein ligase D2) (p53-regulated ubiquitin-conjugating enzyme 1)
Gene: UBE2D2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.82	1.00	0.906

Property	Details
Experiments	12036302, 19690564, 28345603, 36744302
Identification Methods	pull down, affinity chromatography technology, two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0016567 - protein ubiquitination GO:0061630 - ubiquitin protein ligase activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 708 Hs			1 147
+	Hs
		196372 PJA2_HUMAN (1-708): 0: 580: 100.00		UB2D4_HUMAN (1-147): 2.21e-83: 243: 93.20
+	Hs
		196282 PJA2_HUMAN (1-708): 0: 580: 100.00		UB2D1_HUMAN (1-147): 7.06e-83: 241: 89.12
+	Hs
		196289 PJA2_HUMAN (1-708): 0: 580: 100.00		UB2D3_HUMAN (1-147): 1.54e-81: 238: 97.28
+	Hs
		196290 PJA2_HUMAN (1-708): 0: 580: 100.00		UBE2N_HUMAN (4-147): 7.67e-69: 206: 47.22
+	Hs
		919792 PJA1_HUMAN (12-681): 2.38e-128: 396: 42.92		UB2D2_HUMAN (1-147): 9.5e-83: 241: 100.00
+	Hs
		895852 PJA1_HUMAN (12-681): 2.38e-128: 396: 42.92		UB2D3_HUMAN (1-147): 1.54e-81: 238: 97.28
+	Hs
		1245284 PJA1_HUMAN (12-681): 2.38e-128: 396: 42.92		UB2E3_HUMAN (4-146): 3.05e-74: 221: 66.43
+	At
		96605 F4IQ07_ARATH (145-687): 4.23e-61: 217: 14.34		UBC9_ARATH (1-147): 2.75e-82: 239: 80.27
+	At
		96610 F4IQ07_ARATH (145-687): 4.23e-61: 217: 14.34		UBC29_ARATH (1-147): 4.09e-79: 232: 77.55
+	At
		715138 Q8VYL4_ARATH (145-687): 1.69e-56: 203: 14.34		UBC9_ARATH (1-147): 2.75e-82: 239: 80.27
+	At
		1258030 Q8VYL4_ARATH (145-687): 1.69e-56: 203: 14.34		UBC29_ARATH (1-147): 4.09e-79: 232: 77.55
+	Hs
		1346940 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2D4_HUMAN (1-147): 2.21e-83: 243: 93.20
+	Hs
		849459 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2D1_HUMAN (1-147): 7.06e-83: 241: 89.12
+	Hs
		919877 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2D2_HUMAN (1-147): 9.5e-83: 241: 100.00
+	At
		715158 RHG1A_ARATH (207-676): 2.37e-49: 186: 12.01		UBC11_ARATH (1-147): 1.41e-82: 240: 79.59
+	Hs
		895927 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2D3_HUMAN (1-147): 1.54e-81: 238: 97.28
+	At
		715139 RHG1A_ARATH (207-676): 2.37e-49: 186: 12.01		UBC9_ARATH (1-147): 2.75e-82: 239: 80.27
+	Hs
		1297371 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2E2_HUMAN (4-146): 5.85e-75: 223: 65.73
+	Hs
		851893 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2E1_HUMAN (4-146): 8.81e-73: 217: 65.73
+	Hs
		849491 RNF6_HUMAN (67-679): 9.62e-42: 163: 13.13		UB2D1_HUMAN (1-147): 7.06e-83: 241: 89.12