HitPredict

Interactor 1: SH21A (O60880)
SH2 domain-containing protein 1A (Duncan disease SH2-protein) (Signaling lymphocytic activation molecule-associated protein) (SLAM-associated protein) (T-cell signal transduction molecule SAP)
Gene: SH2D1A

Interactor 2: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.58	1.00	0.767

Property	Details
Experiments	25814554, unassigned1126
Identification Methods	two hybrid, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	SH2~SH2, SH2~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0030674 - protein-macromolecule adaptor activity GO:0050860 - negative regulation of T cell receptor signaling pathway
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 128 Hs			1 377
+	Hs
		593935 ABL2_HUMAN (1-127): 1.86e-40: 144: 27.48		NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00
+	Hs
		222027 ABL2_HUMAN (1-127): 1.86e-40: 144: 27.48		NCK2_HUMAN (1-376): 1.95e-92: 283: 68.24
+	Hs
		320220 ABL2_HUMAN (1-127): 1.86e-40: 144: 27.48		SRBS2_HUMAN (64-287): 7.79e-24: 105: 18.26
+	Hs
		440427 ABL2_HUMAN (1-127): 1.86e-40: 144: 27.48		FYN_HUMAN (7-164): 5.43e-24: 105: 17.50
+	Hs
		439898 LCK_HUMAN (1-125): 8.6e-41: 143: 19.08		FYN_HUMAN (7-164): 5.43e-24: 105: 17.50
+	Hs
		473371 HCK_HUMAN (1-127): 5.73e-42: 146: 20.30		P85A_HUMAN (241-376): 5.42e-23: 102: 19.57
+	Hs
		699630 ABL2_HUMAN (1-127): 1.86e-40: 144: 27.48		P85A_HUMAN (241-376): 5.42e-23: 102: 19.57
+	Hs
		473348 HCK_HUMAN (1-127): 5.73e-42: 146: 20.30		HCK_HUMAN (2-190): 3.43e-21: 96.8: 16.84
+	Hs
		136264 HCK_HUMAN (1-127): 5.73e-42: 146: 20.30		P85B_HUMAN (250-376): 7.23e-21: 96.4: 24.03
+	Hs
		439912 LCK_HUMAN (1-125): 8.6e-41: 143: 19.08		HCK_HUMAN (2-190): 3.43e-21: 96.8: 16.84
+	Hs
		136254 LCK_HUMAN (1-125): 8.6e-41: 143: 19.08		P85B_HUMAN (250-376): 7.23e-21: 96.4: 24.03
+	Hs
		473378 HCK_HUMAN (1-127): 5.73e-42: 146: 20.30		ABL2_HUMAN (197-356): 1.15e-15: 80.9: 33.95
+	Hs
		840943 ABL2_HUMAN (1-127): 1.86e-40: 144: 27.48		GRB2_HUMAN (3-61): 2.31e-17: 82.1: 28.81
+	Hs
		840931 ABL2_HUMAN (1-127): 1.86e-40: 144: 27.48		ABL2_HUMAN (197-356): 1.15e-15: 80.9: 33.95
+	Hs
		473361 HCK_HUMAN (1-127): 5.73e-42: 146: 20.30		SRC_HUMAN (5-152): 5.68e-12: 68.8: 19.74
+	Hs
		557158 ABL2_HUMAN (1-127): 1.86e-40: 144: 27.48		SRC_HUMAN (5-152): 5.68e-12: 68.8: 19.74
+	Hs
		439926 LCK_HUMAN (1-125): 8.6e-41: 143: 19.08		SRC_HUMAN (5-152): 5.68e-12: 68.8: 19.74
+	Hs
		439905 LCK_HUMAN (1-125): 8.6e-41: 143: 19.08		YES_HUMAN (112-218): 4.18e-11: 66.1: 19.27
+	Hs
		473458 HCK_HUMAN (1-127): 5.73e-42: 146: 20.30		SH3K1_HUMAN (56-207): 4.07e-09: 60.1: 17.53