HitPredict

Interactor 1: AP2A1 (O95782)
AP-2 complex subunit alpha-1 (100 kDa coated vesicle protein A) (Adaptor protein complex AP-2 subunit alpha-1) (Adaptor-related protein complex 2 subunit alpha-1) (Alpha-adaptin A) (Alpha1-adaptin) (Clathrin assembly protein complex 2 alpha-A large chain) (Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit)
Gene: AP2A1

Interactor 2: NUMB (P49757)
Protein numb homolog (h-Numb) (Protein S171)
Gene: NUMB

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.63	0.60	0.619

Property	Details
Experiments	12942088, 23211419
Identification Methods	affinity chromatography technology, pull down, anti bait coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005886 - plasma membrane GO:0016323 - basolateral plasma membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 977 Hs			1 651
+	Hs
		302120 AP2A2_HUMAN (1-977): 0: 832: 76.97		NUMB_HUMAN (1-651): 7.18e-103: 328: 100.00
+	Rn
		1109842 Q66HM2_RAT (1-977): 0: 845: 79.39		Q3MUI1_RAT (1-651): 2.15e-78: 262: 83.13
+	Rn
		577194 AP2A2_RAT (1-977): 0: 841: 79.18		Q3MUI1_RAT (1-651): 2.15e-78: 262: 83.13
+	Hs
		321528 AP2A1_HUMAN (1-977): 0: 904: 100.00		DAB2_HUMAN (31-643): 9.53e-33: 136: 14.01
+	Mm
		567529 AP2A2_MOUSE (1-977): 0: 841: 79.29		ANS1B_MOUSE (7-213): 4.87e-48: 184: 18.75
+	Hs
		833572 AP2B1_HUMAN (34-972): 0: 676: 17.11		NUMB_HUMAN (1-651): 7.18e-103: 328: 100.00
+	Hs
		833587 AP1B1_HUMAN (34-972): 0: 668: 18.15		NUMB_HUMAN (1-651): 7.18e-103: 328: 100.00
+	Hs
		302137 AP2A2_HUMAN (1-977): 0: 832: 76.97		DAB2_HUMAN (31-643): 9.53e-33: 136: 14.01
+	Dm
		561275 AP2A_DROME (3-977): 0: 784: 66.09		NUMB_DROME (16-540): 1.63e-40: 157: 36.14
+	Rn
		923852 AP2B1_RAT (34-972): 0: 676: 17.11		Q3MUI1_RAT (1-651): 2.15e-78: 262: 83.13
+	Ce
		60774 Q22601_CAEEL (1-977): 0: 768: 60.49		A5JYR4_CAEEL (10-460): 6.7e-37: 146: 14.32
+	Ce
		283751 Q22601_CAEEL (1-977): 0: 768: 60.49		CED6_CAEEL (7-402): 5.6e-36: 143: 14.85
+	Rn
		1144064 Q66HM2_RAT (1-977): 0: 845: 79.39		SYNJ1_RAT (313-650): 6.99e-09: 61.4: 11.77
+	Hs
		925893 AP2B1_HUMAN (34-972): 0: 676: 17.11		ARH_HUMAN (4-293): 1.38e-52: 185: 19.67
+	Hs
		926067 AP2B1_HUMAN (34-972): 0: 676: 17.11		NUMBL_HUMAN (1-651): 4.19e-48: 180: 45.55
+	Hs
		1014028 AP1B1_HUMAN (34-972): 0: 668: 18.15		ARH_HUMAN (4-293): 1.38e-52: 185: 19.67
+	Hs
		925962 AP2B1_HUMAN (34-972): 0: 676: 17.11		ANS1A_HUMAN (8-244): 1.9e-39: 158: 18.49
+	Hs
		253551 AP2B1_HUMAN (34-972): 0: 676: 17.11		CAPON_HUMAN (8-286): 2.51e-35: 141: 17.11
+	Hs
		925851 AP2B1_HUMAN (34-972): 0: 676: 17.11		DAB2_HUMAN (31-643): 9.53e-33: 136: 14.01
+	Ce
		283753 Q9N4F3_CAEEL (34-974): 0: 603: 17.15		CED6_CAEEL (7-402): 5.6e-36: 143: 14.85