HitPredict

Interactor 1: RPB1 (P04050)
DNA-directed RNA polymerase II subunit RPB1 (RNA polymerase II subunit 1) (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit) (RNA polymerase II subunit B220)
Gene: RPO21

Interactor 2: TF2B (P29055)
Transcription initiation factor IIB (General transcription factor TFIIB) (Transcription factor E)
Gene: SUA7

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.90	1.00	0.951

Property	Details
Experiments	17632521, 10464320, 14963322, 37863410, 9651390, 14536083, 23151482
Identification Methods	cross-linking study, pull down, x-ray crystallography, affinity chromatography technology
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	RNA_pol_Rpb1_1~TF_Zn_Ribbon, RNA_pol_Rpb1_2~TF_Zn_Ribbon
Common GO terms	GO:0005634 - nucleus GO:0006367 - transcription initiation at RNA polymerase II promoter GO:0046872 - metal ion binding
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1 1733 Sc			1 345
+	Sc
		375586 RPC1_YEAST (1-1448): 0: 1370: 31.72		TF2B_YEAST (1-345): 4.06e-145: 415: 100.00
+	Hs
		156017 RPC1_HUMAN (1-1457): 0: 1366: 31.97		TF2B_HUMAN (27-340): 2.69e-122: 356: 32.70
+	Sc
		375587 RPC1_YEAST (1-1448): 0: 1370: 31.72		TF3B_YEAST (23-340): 1.67e-85: 271: 16.92
+	Sc
		501331 RPA1_YEAST (5-277): 4.35e-35: 149: 18.31		TF2B_YEAST (1-345): 4.06e-145: 415: 100.00
+	Hs
		624865 RPB1_HUMAN (1529-1719): 2.52e-19: 97.6: 70.23		TF2B_HUMAN (27-340): 2.69e-122: 356: 32.70