HitPredict

Interactor 1: UCHL1 (P09936)
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) (EC 3.4.19.12) (Neuron cytoplasmic protein 9.5) (PGP 9.5) (PGP9.5) (Ubiquitin thioesterase L1)
Gene: UCHL1

Interactor 2: NEDD8 (Q15843)
NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8) (Ubiquitin-like protein Nedd8)
Gene: NEDD8

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	1.00	0.811

Property	Details
Experiments	16169070, 32814053
Identification Methods	two hybrid, two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Peptidase_C12~ubiquitin
Common GO terms	GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 223 Hs			1 81
+	Mm
		669129 UCHL3_MOUSE (2-222): 1.91e-100: 292: 55.31		NEDD8_MOUSE (1-81): 1.24e-41: 132: 98.77
+	Hs
		456536 UCHL1_HUMAN (1-223): 2.52e-103: 299: 100.00		RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14
+	Hs
		456474 UCHL1_HUMAN (1-223): 2.52e-103: 299: 100.00		UBB_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Hs
		474910 UCHL3_HUMAN (2-222): 2.74e-100: 292: 54.43		UBB_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Hs
		548107 UCHL3_HUMAN (2-222): 2.74e-100: 292: 54.43		Q5U5U6_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Hs
		548102 UCHL3_HUMAN (2-222): 2.74e-100: 292: 54.43		NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00
+	Dm
		547969 Q9XZ61_DROME (2-220): 4.78e-91: 272: 22.03		RS27A_DROME (1-77): 3.39e-36: 121: 57.14
+	Hs
		924096 BAP1_HUMAN (3-220): 2.36e-78: 250: 22.27		RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14
+	Dm
		547926 CALYP_DROME (3-220): 1.47e-76: 239: 22.69		RS27A_DROME (1-77): 3.39e-36: 121: 57.14
+	Sp
		145594 UBLH2_SCHPO (1-221): 1.3e-85: 257: 22.71		UBL1_SCHPO (1-76): 9.64e-30: 102: 81.58
+	Sp
		145591 UBLH1_SCHPO (3-220): 1.88e-68: 210: 32.30		UBL1_SCHPO (1-76): 9.64e-30: 102: 81.58
+	Sc
		477613 UBL1_YEAST (5-219): 2.26e-54: 175: 26.29		RL40A_YEAST (1-76): 1.87e-32: 110: 55.26
+	Sc
		408320 UBL1_YEAST (5-219): 2.26e-54: 175: 26.29		RS31_YEAST (1-70): 3.03e-30: 105: 52.86
+	Sc
		715092 UBL1_YEAST (5-219): 2.26e-54: 175: 26.29		RUB1_YEAST (1-76): 1.45e-21: 81.3: 59.21