HitPredict

Interactor 1: ARAF (P10398)
Serine/threonine-protein kinase A-Raf (EC 2.7.11.1) (Proto-oncogene A-Raf) (Proto-oncogene A-Raf-1) (Proto-oncogene Pks)
Gene: ARAF

Interactor 2: 1433B (P31946)
14-3-3 protein beta/alpha (Protein 1054) (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein beta/alpha, N-terminally processed]
Gene: YWHAB

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.972

Property	Details
Experiments	25852190, 29777862, 31980649, 35271311, 32707033, 36931259, 24255178, unassigned2173, unassigned3799
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, pull down
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	14-3-3~C1_1, 14-3-3~PK_Tyr_Ser-Thr, 14-3-3~RBD
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 606 Hs			1 246
+	Hs
		521276 ARAF_HUMAN (1-606): 0: 571: 100.00		1433E_HUMAN (3-243): 4.41e-144: 405: 65.43
+	Hs
		521296 ARAF_HUMAN (1-606): 0: 571: 100.00		1433Z_HUMAN (3-244): 1.49e-141: 398: 87.60
+	Hs
		116544 H7C560_HUMAN (3-604): 0: 539: 55.34		1433E_HUMAN (3-243): 4.41e-144: 405: 65.43
+	Hs
		578439 BRAF_HUMAN (3-604): 0: 537: 54.78		1433E_HUMAN (3-243): 4.41e-144: 405: 65.43
+	Hs
		578401 BRAF_HUMAN (3-604): 0: 537: 54.78		1433B_HUMAN (1-246): 3.49e-144: 404: 100.00
+	Hs
		116545 H7C560_HUMAN (3-604): 0: 539: 55.34		1433Z_HUMAN (3-244): 1.49e-141: 398: 87.60
+	Hs
		578447 BRAF_HUMAN (3-604): 0: 537: 54.78		1433Z_HUMAN (3-244): 1.49e-141: 398: 87.60
+	Hs
		521207 ARAF_HUMAN (1-606): 0: 571: 100.00		1433T_HUMAN (3-244): 3.25e-127: 361: 81.82
+	Gg
		1050125 BRAF_CHICK (3-604): 0: 535: 55.34		1433Z_CHICK (3-244): 7.4e-141: 396: 87.19
+	Gg
		1050127 BRAF_CHICK (3-604): 0: 535: 55.34		1433B_CHICK (3-246): 3.74e-141: 396: 97.95
+	Gg
		1050129 BRAF_CHICK (3-604): 0: 535: 55.34		1433T_CHICK (3-244): 5.64e-128: 363: 82.23
+	Hs
		578385 BRAF_HUMAN (3-604): 0: 537: 54.78		1433T_HUMAN (3-244): 3.25e-127: 361: 81.82
+	Hs
		521270 ARAF_HUMAN (1-606): 0: 571: 100.00		1433G_HUMAN (3-246): 1.92e-109: 317: 75.40
+	Hs
		521322 ARAF_HUMAN (1-606): 0: 571: 100.00		1433F_HUMAN (4-244): 2.72e-107: 311: 75.51
+	Hs
		521221 ARAF_HUMAN (1-606): 0: 571: 100.00		1433S_HUMAN (3-239): 3.4e-100: 293: 68.62
+	Hs
		578437 BRAF_HUMAN (3-604): 0: 537: 54.78		1433G_HUMAN (3-246): 1.92e-109: 317: 75.40
+	Gg
		1050122 BRAF_CHICK (3-604): 0: 535: 55.34		1433G_CHICK (3-246): 1.04e-109: 317: 75.40
+	Hs
		578462 BRAF_HUMAN (3-604): 0: 537: 54.78		1433F_HUMAN (4-244): 2.72e-107: 311: 75.51
+	Gg
		1050126 BRAF_CHICK (3-604): 0: 535: 55.34		Q5ZKJ2_CHICK (4-246): 7.73e-108: 312: 74.90
+	Hs
		578402 BRAF_HUMAN (3-604): 0: 537: 54.78		1433S_HUMAN (3-239): 3.4e-100: 293: 68.62