Interactor 1: BUB3 (P26449)
Spindle assembly checkpoint protein BUB3 (Cell cycle arrest protein BUB3)
Gene: BUB3
Interactor 2: MAD3 (P47074)
Spindle assembly checkpoint component MAD3 (Mitotic MAD3 protein)
Gene: MAD3
Species: Saccharomyces cerevisiae S288C
Source: mint
Category: Small-scale
Confidence: High
| Method score | Annotation score | Interaction score |
|---|---|---|
| 0.95 | 1.00 | 0.978 |
The method score is based on:
| Property | Details |
|---|---|
| Experiments | 17227844, 10688190, 10704439, 17406666, 39079532, 15644329, 18719252, 20498086, 11726501 |
| Identification Methods | isothermal titration calorimetry, molecular sieving, cosedimentation in solution, two hybrid, coimmunoprecipitation, x-ray crystallography, affinity chromatography technology, pull down, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation |
| Interaction Type | direct interaction, physical association, association |
The annotation score is based on:
| Property | Details |
|---|---|
| Interacting Pfam domains | Mad3_BUB1_I~WD40, Mad3_BUB1_II~WD40 |
| Common GO terms | GO:0005634 - nucleus GO:0007094 - mitotic spindle assembly checkpoint signaling GO:0033597 - mitotic checkpoint complex GO:0044774 - mitotic DNA integrity checkpoint signaling GO:1902499 - positive regulation of protein autoubiquitination |
| Homologous interactions | 10 |
Homologous interactions
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Service provided by Combinatics, Japan
Last updated: 11Aug2025
