HitPredict

Interactor 1: RSP5 (P39940)
E3 ubiquitin-protein ligase RSP5 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase RSP5) (Reverses SPT-phenotype protein 5)
Gene: RSP5

Interactor 2: ROG3 (P43602)
Protein ROG3 (Revertant of glycogen synthase kinase mutation protein 3)
Gene: ROG3

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.83	0.60	0.708

Property	Details
Experiments	12163175, 24820415, 17551511, 18976803
Identification Methods	coimmunoprecipitation, affinity chromatography technology, pull down, enzymatic study, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005886 - plasma membrane GO:0070086 - ubiquitin-dependent endocytosis
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 809 Sc			1 733
+	Sc
		765366 RSP5_YEAST (1-809): 0: 825: 100.00		ROD1_YEAST (3-714): 3.11e-177: 530: 39.37
+	Sc
		765309 RSP5_YEAST (1-809): 0: 825: 100.00		ALY2_YEAST (80-704): 1.52e-134: 425: 15.36
+	Sc
		733048 RSP5_YEAST (1-809): 0: 825: 100.00		ALY1_YEAST (86-564): 4.23e-104: 341: 15.63
+	Sp
		1264987 PUB1_SCHPO (4-809): 0: 782: 63.82		ALY1_SCHPO (36-500): 1.56e-121: 375: 17.23
+	Sc
		745491 RSP5_YEAST (1-809): 0: 825: 100.00		ECM21_YEAST (202-686): 6.14e-90: 306: 14.61
+	Sc
		765337 RSP5_YEAST (1-809): 0: 825: 100.00		ART5_YEAST (7-474): 1.19e-93: 305: 21.67
+	Sp
		145423 PUB3_SCHPO (4-809): 0: 733: 54.05		ALY1_SCHPO (36-500): 1.56e-121: 375: 17.23
+	Sc
		765445 RSP5_YEAST (1-809): 0: 825: 100.00		CSR2_YEAST (202-636): 1.73e-67: 243: 14.04
+	Hs
		624998 ITCH_HUMAN (1-809): 0: 727: 34.62		RPB1_HUMAN (569-715): 7.03e-09: 61.6: 13.67
+	Hs
		625052 SMUF1_HUMAN (4-809): 0: 701: 36.64		RPB1_HUMAN (569-715): 7.03e-09: 61.6: 13.67
+	Mm
		444988 WWP2_MOUSE (107-809): 0: 681: 41.35		RPB1_MOUSE (582-705): 1.29e-13: 77: 12.10
+	Hs
		625031 WWP1_HUMAN (108-809): 0: 690: 40.33		RPB1_HUMAN (569-715): 7.03e-09: 61.6: 13.67
+	Hs
		125161 WWP2_HUMAN (100-809): 0: 684: 40.81		RPB1_HUMAN (569-715): 7.03e-09: 61.6: 13.67
+	Hs
		624804 NEDD4_HUMAN (102-807): 0: 668: 39.67		RPB1_HUMAN (569-715): 7.03e-09: 61.6: 13.67
+	Sc
		758550 TOM1_YEAST (370-808): 5.87e-157: 509: 37.36		SEC31_YEAST (391-716): 2.57e-10: 66.2: 10.65
+	Hs
		1170093 HECW2_HUMAN (10-413): 1.88e-09: 63.7: 12.12		SRCAP_HUMAN (376-725): 9.48e-09: 61.2: 15.98