HitPredict

Interactor 1: AFAD (P55196)
Afadin (ALL1-fused gene from chromosome 6 protein) (Protein AF-6) (Afadin adherens junction formation factor)
Gene: AFDN

Interactor 2: SRBS1 (Q9BX66)
Sorbin and SH3 domain-containing protein 1 (Ponsin) (SH3 domain protein 5) (SH3P12) (c-Cbl-associated protein) (CAP)
Gene: SORBS1

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.64	1.00	0.806

Property	Details
Experiments	10085297, 34079125
Identification Methods	affinity chromatography technology, pull down, proximity labelling technology
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	PDZ~SH3_2
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0005912 - adherens junction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1824 Hs			1 1292
+	Hs
		209019 AFAD_HUMAN (1-1824): 0: 1279: 100.00		NCK2_HUMAN (767-1019): 1.85e-42: 162: 16.23
+	Hs
		876420 AFAD_HUMAN (1-1824): 0: 1279: 100.00		DNMBP_HUMAN (796-1253): 2.23e-17: 90.7: 14.23
+	Hs
		298964 AFAD_HUMAN (1-1824): 0: 1279: 100.00		SRBS2_HUMAN (516-926): 8.23e-14: 78.7: 14.74
+	Hs
		235492 AFAD_HUMAN (1-1824): 0: 1279: 100.00		VINEX_HUMAN (852-938): 9.3e-09: 61.9: 27.59
+	Hs
		1066628 KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67		SRBS1_HUMAN (1-1292): 5.5e-176: 558: 100.00
+	Hs
		1045417 KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67		SPTN1_HUMAN (304-902): 8.17e-59: 225: 13.39
+	Hs
		557789 KI13A_HUMAN (184-691): 2.35e-70: 264: 13.74		NCK1_HUMAN (708-1005): 1.37e-49: 182: 18.27
+	Mm
		1143326 DLG4_MOUSE (1002-1238): 8.46e-28: 123: 17.51		SRBS1_MOUSE (1-1292): 3.51e-71: 263: 20.24
+	Hs
		117900 KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67		MYO1F_HUMAN (1104-1290): 4.97e-28: 124: 18.72
+	Hs
		298003 GOPC_HUMAN (744-1099): 5.55e-40: 157: 14.72		FCSD2_HUMAN (340-994): 3.64e-50: 192: 11.44
+	Mm
		561163 DLG4_MOUSE (1002-1238): 8.46e-28: 123: 17.51		SPTN1_MOUSE (304-921): 8.47e-58: 222: 13.46
+	Hs
		1021662 KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67		MYO1E_HUMAN (1089-1290): 2.99e-25: 116: 16.06
+	Hs
		1052200 KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67		SRC8_HUMAN (991-1288): 1.92e-23: 108: 13.38
+	Hs
		1256281 KI13B_HUMAN (184-691): 5.1e-66: 250: 12.64		SH3R3_HUMAN (407-924): 1.49e-12: 74.3: 10.82
+	Hs
		298982 KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67		SRBS2_HUMAN (516-926): 8.23e-14: 78.7: 14.74
+	Mm
		223014 DLG4_MOUSE (1002-1238): 8.46e-28: 123: 17.51		NCK2_MOUSE (761-1019): 5.71e-43: 163: 16.61
+	Hs
		1052346 NEB1_HUMAN (1002-1092): 1.04e-19: 98.7: 40.00		SRC8_HUMAN (991-1288): 1.92e-23: 108: 13.38
+	Mm
		1113245 DLG4_MOUSE (1002-1238): 8.46e-28: 123: 17.51		SRBS2_MOUSE (508-926): 2.95e-15: 83.3: 15.18
+	Mm
		1143413 DLG4_MOUSE (1002-1238): 8.46e-28: 123: 17.51		SH3R3_MOUSE (516-848): 7.8e-11: 68.9: 11.87
+	Dm
		113458 M9NFI9_DROME (1002-1092): 6.19e-22: 106: 38.95		Q9VIF7_DROME (593-865): 1.5e-13: 77.9: 13.04