HitPredict

Interactor 1: SUMO3 (P55854)
Small ubiquitin-related modifier 3 (SUMO-3) (SMT3 homolog 1) (SUMO-2) (Ubiquitin-like protein SMT3A) (Smt3A)
Gene: SUMO3

Interactor 2: UBC9 (P63279)
SUMO-conjugating enzyme UBC9 (EC 2.3.2.-) (RING-type E3 SUMO transferase UBC9) (SUMO-protein ligase) (Ubiquitin carrier protein 9) (Ubiquitin carrier protein I) (Ubiquitin-conjugating enzyme E2 I) (Ubiquitin-protein ligase I) (p18)
Gene: UBE2I

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.965

Property	Details
Experiments	11451954, 28514442, 17353931, 33961781, 16620772, 21949651, 16524884
Identification Methods	enzymatic study, affinity chromatography technology, two hybrid, pull down, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	Rad60-SLD~UQ_con
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0016605 - PML body GO:0016925 - protein sumoylation
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 103 Hs			1 158
+	Mm
		930505 SUMO3_MOUSE (1-97): 4.76e-46: 145: 94.85		UBC9_MOUSE (1-158): 1.43e-70: 211: 100.00
+	Rn
		930510 SUMO3_RAT (1-97): 6.47e-45: 142: 94.85		UBC9_RAT (1-158): 1.43e-70: 211: 100.00
+	At
		1114967 SUMO2_ARATH (1-98): 1.43e-41: 133: 47.96		SCE1_ARATH (2-155): 5.4e-72: 215: 66.23
+	Hs
		902509 SUMO2_HUMAN (1-93): 4.15e-41: 132: 96.81		UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00
+	At
		1114966 SUMO3_ARATH (1-96): 2.22e-37: 123: 40.21		SCE1_ARATH (2-155): 5.4e-72: 215: 66.23
+	Hs
		928121 SUMO1_HUMAN (1-92): 2.47e-36: 120: 48.45		Q7KZS0_HUMAN (1-137): 2.27e-71: 214: 100.00
+	At
		878960 SUMO1_ARATH (2-90): 1.01e-35: 118: 47.19		SCE1_ARATH (2-155): 5.4e-72: 215: 66.23
+	Hs
		102167 SUMO5_HUMAN (1-92): 1.21e-35: 118: 46.39		Q7KZS0_HUMAN (1-137): 2.27e-71: 214: 100.00
+	Hs
		928056 SUMO1_HUMAN (1-92): 2.47e-36: 120: 48.45		UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00
+	Mm
		928242 SUMO1_MOUSE (1-92): 2.47e-36: 120: 48.45		UBC9_MOUSE (1-158): 1.43e-70: 211: 100.00
+	Hs
		102156 SUMO5_HUMAN (1-92): 1.21e-35: 118: 46.39		UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00
+	Sp
		140897 PMT3_SCHPO (2-92): 2.5e-39: 128: 50.55		UBC9_SCHPO (1-157): 1.37e-66: 201: 66.24
+	Dm
		332535 O97102_DROME (6-94): 4.45e-39: 127: 69.66		Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44
+	Ce
		879079 SUMO_CAEEL (1-92): 8.91e-35: 116: 43.48		UBC9_CAEEL (1-157): 3.2e-68: 205: 76.43
+	Zm
		74333 B6TQW3_MAIZE (3-90): 2.25e-26: 98.9: 30.68		B4FC97_MAIZE (4-157): 8.79e-75: 221: 35.71
+	At
		1114930 Q8LCF9_ARATH (2-90): 1.41e-26: 98.9: 26.67		SCE1_ARATH (2-155): 5.4e-72: 215: 66.23
+	Mm
		140343 NF2IP_MOUSE (5-85): 7.2e-24: 95.1: 28.05		UBC9_MOUSE (1-158): 1.43e-70: 211: 100.00
+	Sc
		841454 SMT3_YEAST (2-93): 3.78e-32: 109: 44.57		UBC9_YEAST (1-156): 3.53e-61: 187: 56.41