HitPredict

Interactor 1: 1433E (P62258)
14-3-3 protein epsilon (14-3-3E)
Gene: YWHAE

Interactor 2: NEK1 (Q96PY6)
Serine/threonine-protein kinase Nek1 (EC 2.7.11.1) (Never in mitosis A-related kinase 1) (NimA-related protein kinase 1) (Renal carcinoma antigen NY-REN-55)
Gene: NEK1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	0.60	0.611

Property	Details
Experiments	35271311, 36931259
Identification Methods	affinity chromatography technology, proximity labelling technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 255 Hs			1 1258
+	Hs
		909658 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MRCKA_HUMAN (2-901): 2.99e-144: 480: 19.19
+	Hs
		909524 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		ROCK1_HUMAN (2-1044): 4.42e-142: 467: 15.73
+	Hs
		909916 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK4_HUMAN (3-644): 2.23e-138: 441: 16.56
+	Mm
		910231 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK3_MOUSE (3-645): 1.73e-138: 441: 17.07
+	Hs
		647862 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK3_HUMAN (3-645): 1.72e-137: 439: 17.22
+	Hs
		909730 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK2_HUMAN (3-645): 3.79e-137: 439: 17.42
+	Mm
		910308 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK4_MOUSE (3-644): 1.03e-137: 439: 17.31
+	Mm
		910234 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK2_MOUSE (3-645): 9.66e-137: 437: 17.26
+	Hs
		910013 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		SIK2_HUMAN (4-781): 8.83e-134: 434: 15.15
+	Xl
		223345 O57468_XENLA (1-255): 1.7e-152: 426: 96.47		Q8QGV3_XENLA (3-645): 1.86e-136: 437: 17.67
+	Xl
		223344 O57468_XENLA (1-255): 1.7e-152: 426: 96.47		Q804T2_XENLA (3-645): 4.31e-133: 426: 16.15
+	Hs
		871354 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		AAPK2_HUMAN (3-508): 9.05e-125: 398: 17.46
+	Hs
		681770 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		AKT1_HUMAN (1-316): 5.31e-119: 380: 27.41
+	At
		965360 14332_ARATH (4-255): 1.05e-147: 414: 74.41		CIPKC_ARATH (3-386): 2.25e-120: 384: 22.68
+	At
		788672 14333_ARATH (1-253): 3.45e-147: 413: 74.12		CIPKC_ARATH (3-386): 2.25e-120: 384: 22.68
+	At
		1280891 14337_ARATH (2-255): 7.73e-147: 412: 71.21		CIPKC_ARATH (3-386): 2.25e-120: 384: 22.68
+	Hs
		909532 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		KCC2D_HUMAN (2-281): 1.99e-112: 363: 28.82
+	Hs
		851417 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		NEK4_HUMAN (1-266): 1.31e-78: 279: 50.56
+	Hs
		909842 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		NEK9_HUMAN (4-261): 3.69e-61: 229: 42.31
+	Hs
		909590 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		KS6A2_HUMAN (8-274): 4.38e-19: 95.5: 25.45