HitPredict

Interactor 1: GNAI1 (P63096)
Guanine nucleotide-binding protein G(i) subunit alpha-1 (Adenylate cyclase-inhibiting G alpha protein)
Gene: GNAI1

Interactor 2: GBG5 (P63218)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-5
Gene: GNG5

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.60	0.667

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0003924 - GTPase activity GO:0005834 - heterotrimeric G-protein complex GO:0005886 - plasma membrane GO:0007186 - G protein-coupled receptor signaling pathway GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 354 Hs			1 68
+	Hs
		229239 GNAI1_HUMAN (1-354): 0: 512: 100.00		GBG7_HUMAN (1-68): 1.09e-27: 96.2: 51.47
+	Hs
		887099 GNAI1_HUMAN (1-354): 0: 512: 100.00		GBG2_HUMAN (2-68): 6.55e-26: 91.6: 48.53
+	Hs
		926667 GNAI1_HUMAN (1-354): 0: 512: 100.00		GBG3_HUMAN (1-68): 1.3e-25: 90.9: 46.38
+	Hs
		837689 GNAI1_HUMAN (1-354): 0: 512: 100.00		GBG10_HUMAN (1-68): 5.93e-25: 89.3: 52.94
+	Hs
		837675 GNAI1_HUMAN (1-354): 0: 512: 100.00		GBG4_HUMAN (2-68): 1.64e-24: 88.2: 45.59
+	Hs
		229234 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		GBG7_HUMAN (1-68): 1.09e-27: 96.2: 51.47
+	Hs
		926724 GNAI1_HUMAN (1-354): 0: 512: 100.00		GBG8_HUMAN (2-68): 1.13e-23: 85.8: 44.12
+	Hs
		229233 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		GBG7_HUMAN (1-68): 1.09e-27: 96.2: 51.47
+	Hs
		444709 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		GBG3_HUMAN (1-68): 1.3e-25: 90.9: 46.38
+	Hs
		444695 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		GBG10_HUMAN (1-68): 5.93e-25: 89.3: 52.94
+	Hs
		444694 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		GBG4_HUMAN (2-68): 1.64e-24: 88.2: 45.59
+	Hs
		391364 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		GBG2_HUMAN (2-68): 6.55e-26: 91.6: 48.53
+	Hs
		391377 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		GBG3_HUMAN (1-68): 1.3e-25: 90.9: 46.38
+	Hs
		444710 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		GBG5_HUMAN (1-68): 5.15e-24: 86.6: 100.00
+	Hs
		391360 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		GBG10_HUMAN (1-68): 5.93e-25: 89.3: 52.94
+	Hs
		391359 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		GBG4_HUMAN (2-68): 1.64e-24: 88.2: 45.59
+	Hs
		391378 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		GBG5_HUMAN (1-68): 5.15e-24: 86.6: 100.00
+	Hs
		391482 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		GBG8_HUMAN (2-68): 1.13e-23: 85.8: 44.12
+	Hs
		450990 GNAO_HUMAN (1-354): 4.45e-175: 491: 72.11		GBG10_HUMAN (1-68): 5.93e-25: 89.3: 52.94
+	Hs
		444701 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		GBG11_HUMAN (9-67): 1.67e-18: 73.1: 32.20