HitPredict

Interactor 1: SMAD5 (Q99717)
Mothers against decapentaplegic homolog 5 (MAD homolog 5) (Mothers against DPP homolog 5) (JV5-1) (SMAD family member 5) (SMAD 5) (Smad5) (hSmad5)
Gene: SMAD5

Interactor 2: SMUF1 (Q9HCE7)
E3 ubiquitin-protein ligase SMURF1 (hSMURF1) (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase SMURF1) (SMAD ubiquitination regulatory factor 1) (SMAD-specific E3 ubiquitin-protein ligase 1)
Gene: SMURF1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	0.60	0.754

Property	Details
Experiments	18641638, 11278251, 20484049, 19914168, 22670624, 17676934, 15221015, 12857866, 16516194, 15231748
Identification Methods	enzymatic study, affinity chromatography technology, pull down, unspecified method, two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0030154 - cell differentiation GO:0030509 - BMP signaling pathway and 1 others more... GO:0070411 - I-SMAD binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 465 Hs			1 757
+	Hs
		1078761 SMAD1_HUMAN (3-465): 0: 637: 89.68		SMUF1_HUMAN (1-757): 0: 891: 100.00
+	Mm
		936618 SMAD1_MOUSE (3-465): 0: 638: 89.89		SMUF1_MOUSE (1-757): 0: 855: 95.24
+	Xl
		945613 P79947_XENLA (3-465): 0: 643: 89.87		SMUF1_XENLA (1-757): 0: 826: 87.07
+	Hs
		1305886 SMAD5_HUMAN (1-465): 0: 650: 100.00		SMUF2_HUMAN (1-757): 0: 794: 70.72
+	Hs
		950291 SMAD3_HUMAN (4-465): 0: 550: 58.97		SMUF1_HUMAN (1-757): 0: 891: 100.00
+	Mm
		56826 SMAD1_MOUSE (3-465): 0: 638: 89.89		SMUF2_MOUSE (1-757): 0: 795: 71.30
+	Hs
		1078760 SMAD1_HUMAN (3-465): 0: 637: 89.68		SMUF2_HUMAN (1-757): 0: 794: 70.72
+	Hs
		950287 SMAD3_HUMAN (4-465): 0: 550: 58.97		SMUF2_HUMAN (1-757): 0: 794: 70.72
+	Hs
		808240 SMAD5_HUMAN (1-465): 0: 650: 100.00		NEDD4_HUMAN (93-755): 0: 673: 36.63
+	Hs
		808135 SMAD1_HUMAN (3-465): 0: 637: 89.68		NEDD4_HUMAN (93-755): 0: 673: 36.63
+	Hs
		950248 SMAD3_HUMAN (4-465): 0: 550: 58.97		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		125369 SMAD3_HUMAN (4-465): 0: 550: 58.97		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		808087 SMAD3_HUMAN (4-465): 0: 550: 58.97		NEDD4_HUMAN (93-755): 0: 673: 36.63
+	Hs
		1305881 SMAD5_HUMAN (1-465): 0: 650: 100.00		WWP1_HUMAN (2-395): 1.65e-27: 121: 14.94
+	Hs
		1078757 SMAD1_HUMAN (3-465): 0: 637: 89.68		WWP1_HUMAN (2-395): 1.65e-27: 121: 14.94
+	Hs
		1305892 SMAD5_HUMAN (1-465): 0: 650: 100.00		HECW2_HUMAN (1-322): 1.89e-15: 82.9: 14.87
+	Dm
		785477 MAD_DROME (1-465): 0: 596: 72.47		SMUF1_DROME (5-293): 2.09e-29: 127: 34.24
+	Dm
		785516 MAD_DROME (1-465): 0: 596: 72.47		SUDX_DROME (14-413): 1.29e-24: 112: 15.47
+	Hs
		950249 SMAD3_HUMAN (4-465): 0: 550: 58.97		NED4L_HUMAN (6-396): 7.88e-36: 147: 16.88
+	Hs
		950278 SMAD3_HUMAN (4-465): 0: 550: 58.97		WWP1_HUMAN (2-395): 1.65e-27: 121: 14.94