HitPredict

Interactor 1: TNIK (Q9UKE5)
TRAF2 and NCK-interacting protein kinase (EC 2.7.11.1)
Gene: TNIK

Interactor 2: MACF1 (Q9UPN3)
Microtubule-actin cross-linking factor 1, isoforms 1/2/3/4/5 (620 kDa actin-binding protein) (ABP620) (Actin cross-linking family protein 7) (Macrophin-1) (Trabeculin-alpha)
Gene: MACF1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.55	1.00	0.743

Property	Details
Experiments	17043677, unassigned1973
Identification Methods	two hybrid, two hybrid fragment pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	EF-hand_7~Pkinase
Common GO terms	GO:0005737 - cytoplasm GO:0005856 - cytoskeleton GO:0016055 - Wnt signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1360 Hs			1 7388
+	Mm
		949420 TNIK_MOUSE (1-1360): 0: 1208: 96.10		MACF1_MOUSE (1-7388): 0: 2164: 83.39
+	Hs
		1071717 KS6A2_HUMAN (10-709): 1.39e-140: 449: 18.52		MACF1_HUMAN (1-7388): 0: 2387: 100.00
+	Hs
		1357666 MAST1_HUMAN (2-1051): 2.7e-124: 424: 14.80		MACF1_HUMAN (1-7388): 0: 2387: 100.00
+	Hs
		647985 MARK3_HUMAN (3-675): 1.72e-124: 406: 16.37		MACF1_HUMAN (1-7388): 0: 2387: 100.00
+	Hs
		1244144 TNIK_HUMAN (1-1360): 0: 1235: 100.00		SYNE1_HUMAN (343-1742): 3.05e-13: 80.2: 8.97
+	Hs
		974168 TNIK_HUMAN (1-1360): 0: 1235: 100.00		DYST_HUMAN (478-1511): 2.31e-12: 77.1: 9.72
+	Mm
		949395 TNIK_MOUSE (1-1360): 0: 1208: 96.10		DYST_MOUSE (343-1742): 6.98e-20: 102: 9.92
+	Hs
		963449 TNIK_HUMAN (1-1360): 0: 1235: 100.00		SPTB2_HUMAN (6378-7134): 6.05e-11: 72.5: 13.80
+	Mm
		949304 TNIK_MOUSE (1-1360): 0: 1208: 96.10		SPTB2_MOUSE (6378-7134): 3.94e-11: 72.8: 13.67
+	Mm
		949310 TNIK_MOUSE (1-1360): 0: 1208: 96.10		Q68FG2_MOUSE (330-850): 2.48e-10: 70.5: 12.57
+	Mm
		949333 TNIK_MOUSE (1-1360): 0: 1208: 96.10		SYNE1_MOUSE (332-1497): 1.5e-09: 67.8: 10.31
+	Mm
		561157 TNIK_MOUSE (1-1360): 0: 1208: 96.10		SPTN1_MOUSE (433-1344): 2.66e-09: 66.8: 14.03
+	Mm
		82755 TNIK_MOUSE (1-1360): 0: 1208: 96.10		B9EKJ1_MOUSE (433-1344): 4.47e-09: 66.1: 13.69
+	Hs
		885258 MRCKA_HUMAN (12-1330): 0: 690: 13.75		EPIPL_HUMAN (1538-2789): 1.49e-35: 154: 13.15
+	Mm
		93559 TAOK2_MOUSE (1-858): 2.53e-132: 440: 22.14		DESP_MOUSE (2737-3805): 1.11e-27: 127: 8.89
+	Hs
		885239 STK4_HUMAN (5-482): 7.72e-121: 387: 33.06		EPIPL_HUMAN (1538-2789): 1.49e-35: 154: 13.15
+	Hs
		1329044 KS6A6_HUMAN (10-709): 1.13e-130: 422: 16.27		SPTN4_HUMAN (4289-5023): 8.23e-09: 65.5: 19.24
+	Hs
		521610 SIK2_HUMAN (15-915): 3.97e-124: 410: 14.70		ACTN1_HUMAN (921-1398): 1.47e-10: 70.5: 9.07
+	Hs
		647842 MARK3_HUMAN (3-675): 1.72e-124: 406: 16.37		UTRN_HUMAN (428-1437): 8.81e-11: 71.9: 13.98
+	Hs
		1023039 STK4_HUMAN (5-482): 7.72e-121: 387: 33.06		SPTN1_HUMAN (433-1344): 7.43e-10: 68.8: 13.39