HitPredict

Interactor 1: ASAP1 (Q9ULH1)
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 (130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein) (ADP-ribosylation factor-directed GTPase-activating protein 1) (ARF GTPase-activating protein 1) (Development and differentiation-enhancing factor 1) (DEF-1) (Differentiation-enhancing factor 1) (PIP2-dependent ARF1 GAP)
Gene: ASAP1

Interactor 2: CD2AP (Q9Y5K6)
CD2-associated protein (Adapter protein CMS) (Cas ligand with multiple SH3 domains)
Gene: CD2AP

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.55	1.00	0.743

Property	Details
Experiments	15632162, unassigned1973
Identification Methods	affinity chromatography technology, two hybrid fragment pooling approach
Interaction Type	physical association

Property	Details
Interacting Pfam domains	Ank_2~SH3_1, Ank_2~SH3_9, PH~SH3_1, PH~SH3_9, SH3_9~SH3_9
Common GO terms	GO:0002102 - podosome GO:0032588 - trans-Golgi network membrane GO:0045296 - cadherin binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1129 Hs			1 639
+	Hs
		1285221 ASAP1_HUMAN (17-1129): 0: 831: 100.00		SH3K1_HUMAN (1-638): 3.71e-91: 297: 32.43
+	Hs
		209187 ASAP1_HUMAN (17-1129): 0: 831: 100.00		NCK2_HUMAN (2-361): 2.2e-59: 205: 16.17
+	Hs
		275240 ASAP1_HUMAN (17-1129): 0: 831: 100.00		GRAP2_HUMAN (2-329): 1.68e-45: 166: 12.83
+	Hs
		924943 ASAP1_HUMAN (17-1129): 0: 831: 100.00		GRB2_HUMAN (112-328): 1.9e-40: 148: 18.27
+	Hs
		195796 ASAP2_HUMAN (21-967): 0: 647: 55.31		Q5JPT6_HUMAN (1-638): 3.71e-91: 297: 32.43
+	Hs
		195804 ASAP2_HUMAN (21-967): 0: 647: 55.31		SH3K1_HUMAN (1-638): 3.71e-91: 297: 32.43
+	Hs
		557823 ASAP1_HUMAN (17-1129): 0: 831: 100.00		NCK1_HUMAN (113-378): 9.79e-21: 96.5: 14.24
+	Hs
		195770 ASAP2_HUMAN (21-967): 0: 647: 55.31		NCK2_HUMAN (2-361): 2.2e-59: 205: 16.17
+	Hs
		195791 ASAP2_HUMAN (21-967): 0: 647: 55.31		GRB2_HUMAN (112-328): 1.9e-40: 148: 18.27
+	Hs
		195783 ASAP2_HUMAN (21-967): 0: 647: 55.31		NCK1_HUMAN (113-378): 9.79e-21: 96.5: 14.24
+	Hs
		195795 ASAP2_HUMAN (21-967): 0: 647: 55.31		ITSN1_HUMAN (1-55): 3.97e-11: 68.8: 43.64
+	Hs
		924582 ACAP1_HUMAN (45-690): 2.77e-153: 477: 21.01		GRB2_HUMAN (112-328): 1.9e-40: 148: 18.27
+	Hs
		557696 ACAP1_HUMAN (45-690): 2.77e-153: 477: 21.01		NCK1_HUMAN (113-378): 9.79e-21: 96.5: 14.24
+	Hs
		209145 ARAP1_HUMAN (368-620): 3.83e-58: 222: 24.90		NCK2_HUMAN (2-361): 2.2e-59: 205: 16.17
+	Mm
		1112583 AGAP2_MOUSE (379-660): 1.64e-87: 309: 29.02		SRBS1_MOUSE (58-326): 2.72e-19: 94.7: 17.93
+	Hs
		253433 AGAP1_HUMAN (361-662): 1.75e-62: 231: 28.30		SRGP2_HUMAN (2-443): 3.56e-43: 169: 11.52
+	Mm
		1112566 AGAP2_MOUSE (379-660): 1.64e-87: 309: 29.02		SRBS2_MOUSE (258-329): 5.28e-09: 61.5: 20.83
+	Hs
		924798 ARAP1_HUMAN (368-620): 3.83e-58: 222: 24.90		GRB2_HUMAN (112-328): 1.9e-40: 148: 18.27
+	Hs
		557775 ARAP1_HUMAN (368-620): 3.83e-58: 222: 24.90		NCK1_HUMAN (113-378): 9.79e-21: 96.5: 14.24
+	Hs
		235575 ARAP1_HUMAN (368-620): 3.83e-58: 222: 24.90		VINEX_HUMAN (163-343): 4.52e-13: 74.2: 18.28