HitPredict

Interactor 1: OTUB1 (Q96FW1)
Ubiquitin thioesterase OTUB1 (EC 3.4.19.12) (Deubiquitinating enzyme OTUB1) (OTU domain-containing ubiquitin aldehyde-binding protein 1) (Otubain-1) (hOTU1) (Ubiquitin-specific-processing protease OTUB1)
Gene: OTUB1

Interactor 2: UB2D4 (Q9Y2X8)
Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)
Gene: UBE2D4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.86	0.80	0.833

Property	Details
Experiments	25416956, 32296183, 33961781, 31515488, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, two hybrid array, validated two hybrid, two hybrid prey pooling approach, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	Peptidase_C65~UQ_con
Common GO terms	-
Homologous interactions	10

Homologous interactions

Color coding for hit scores

	1 271 Hs			1 147
+	Hs
		906745 OTUB1_HUMAN (1-271): 3.26e-150: 422: 100.00		UB2D1_HUMAN (1-147): 3.33e-83: 242: 91.84
+	Hs
		982284 OTUB1_HUMAN (1-271): 3.26e-150: 422: 100.00		UB2D2_HUMAN (1-147): 7.67e-83: 241: 93.20
+	Hs
		957165 OTUB1_HUMAN (1-271): 3.26e-150: 422: 100.00		UB2D3_HUMAN (1-147): 8.57e-82: 238: 91.84
+	Hs
		1391011 OTUB1_HUMAN (1-271): 3.26e-150: 422: 100.00		UB2E2_HUMAN (4-146): 2.83e-75: 224: 64.34
+	Hs
		1381181 OTUB1_HUMAN (1-271): 3.26e-150: 422: 100.00		UB2E3_HUMAN (4-146): 1.06e-74: 223: 64.34
+	Hs
		909372 OTUB1_HUMAN (1-271): 3.26e-150: 422: 100.00		UB2E1_HUMAN (4-146): 3.9e-73: 218: 64.34
+	Hs
		957818 OTUB1_HUMAN (1-271): 3.26e-150: 422: 100.00		UBE2N_HUMAN (4-147): 2.31e-69: 207: 46.53
+	Mm
		957885 OTUB1_MOUSE (1-271): 3.19e-150: 422: 99.63		UBE2N_MOUSE (4-147): 2.31e-69: 207: 46.53
+	Ce
		762745 OTUBL_CAEEL (3-271): 2.77e-122: 352: 34.42		UBC2_CAEEL (1-147): 6.88e-83: 241: 92.52
+	Hs
		957811 OTUB2_HUMAN (47-270): 1.26e-104: 305: 47.77		UBE2N_HUMAN (4-147): 2.31e-69: 207: 46.53