HitPredict

Interactor 1: TRI18 (O15344)
E3 ubiquitin-protein ligase Midline-1 (EC 2.3.2.27) (Midin) (Putative transcription factor XPRF) (RING finger protein 59) (RING finger protein Midline-1) (RING-type E3 ubiquitin transferase Midline-1) (Tripartite motif-containing protein 18)
Gene: MID1

Interactor 2: UB2D3 (P61077)
Ubiquitin-conjugating enzyme E2 D3 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D3) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D3) (Ubiquitin carrier protein D3) (Ubiquitin-conjugating enzyme E2(17)KB 3) (Ubiquitin-conjugating enzyme E2-17 kDa 3) (Ubiquitin-protein ligase D3)
Gene: UBE2D3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.978

Property	Details
Experiments	19549727, 21143188, 25207814, 25278022, 25416956, 28760657, 32296183, 22613722, unassigned1304, 31515488
Identification Methods	two hybrid, pull down, affinity chromatography technology, validated two hybrid, two hybrid prey pooling approach, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 667 Hs			1 147
+	Hs
		186599 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2D4_HUMAN (1-147): 8.97e-84: 243: 91.84
+	Hs
		186519 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2D1_HUMAN (1-147): 3.03e-83: 242: 88.44
+	Hs
		186530 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2D2_HUMAN (1-147): 4.03e-83: 242: 97.28
+	Hs
		186577 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2E2_HUMAN (4-146): 2.35e-75: 224: 65.04
+	Hs
		186570 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2E3_HUMAN (4-146): 1.4e-74: 222: 65.73
+	Hs
		186520 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2E1_HUMAN (4-146): 3.07e-73: 218: 65.04
+	Hs
		1233123 Q6GX22_HUMAN (1-665): 9.33e-171: 505: 76.24		UB2D4_HUMAN (1-147): 8.97e-84: 243: 91.84
+	Hs
		186526 TRI18_HUMAN (1-667): 0: 536: 100.00		UBE2N_HUMAN (4-147): 5.95e-69: 206: 46.53
+	Hs
		1460665 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2D4_HUMAN (1-147): 8.97e-84: 243: 91.84
+	Hs
		906818 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2D1_HUMAN (1-147): 3.03e-83: 242: 88.44
+	Hs
		982356 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2D2_HUMAN (1-147): 4.03e-83: 242: 97.28
+	Hs
		957232 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2D3_HUMAN (1-147): 6.9e-82: 239: 100.00
+	Hs
		1397303 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2E2_HUMAN (4-146): 2.35e-75: 224: 65.04
+	Hs
		1381218 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2E3_HUMAN (4-146): 1.4e-74: 222: 65.73
+	Hs
		957760 Q6GX22_HUMAN (1-665): 9.33e-171: 505: 76.24		UBE2N_HUMAN (4-147): 5.95e-69: 206: 46.53
+	Hs
		909409 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2E1_HUMAN (4-146): 3.07e-73: 218: 65.04
+	Hs
		957862 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UBE2N_HUMAN (4-147): 5.95e-69: 206: 46.53
+	Hs
		906666 TRI25_HUMAN (4-656): 1.55e-105: 335: 23.57		UB2D1_HUMAN (1-147): 3.03e-83: 242: 88.44
+	Hs
		957079 TRI25_HUMAN (4-656): 1.55e-105: 335: 23.57		UB2D3_HUMAN (1-147): 6.9e-82: 239: 100.00
+	Hs
		957737 TRI25_HUMAN (4-656): 1.55e-105: 335: 23.57		UBE2N_HUMAN (4-147): 5.95e-69: 206: 46.53