HitPredict

Interactor 1: TRI18 (O15344)
E3 ubiquitin-protein ligase Midline-1 (EC 2.3.2.27) (Midin) (Putative transcription factor XPRF) (RING finger protein 59) (RING finger protein Midline-1) (RING-type E3 ubiquitin transferase Midline-1) (Tripartite motif-containing protein 18)
Gene: MID1

Interactor 2: UB2D4 (Q9Y2X8)
Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)
Gene: UBE2D4

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.80	0.80	0.801

Property	Details
Experiments	32296183, 19549727, 21143188, unassigned1304
Identification Methods	two hybrid, validated two hybrid, two hybrid array, two hybrid prey pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	-
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 667 Hs			1 147
+	Hs
		186519 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2D1_HUMAN (1-147): 3.33e-83: 242: 91.84
+	Hs
		186530 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2D2_HUMAN (1-147): 7.67e-83: 241: 93.20
+	Hs
		186524 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2D3_HUMAN (1-147): 8.57e-82: 238: 91.84
+	Hs
		186577 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2E2_HUMAN (4-146): 2.83e-75: 224: 64.34
+	Hs
		186570 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2E3_HUMAN (4-146): 1.06e-74: 223: 64.34
+	Hs
		186520 TRI18_HUMAN (1-667): 0: 536: 100.00		UB2E1_HUMAN (4-146): 3.9e-73: 218: 64.34
+	Hs
		1233123 Q6GX22_HUMAN (1-665): 9.33e-171: 505: 76.24		UB2D4_HUMAN (1-147): 4.38e-84: 244: 100.00
+	Hs
		186526 TRI18_HUMAN (1-667): 0: 536: 100.00		UBE2N_HUMAN (4-147): 2.31e-69: 207: 46.53
+	Hs
		1460665 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2D4_HUMAN (1-147): 4.38e-84: 244: 100.00
+	Hs
		906818 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2D1_HUMAN (1-147): 3.33e-83: 242: 91.84
+	Hs
		982356 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2D2_HUMAN (1-147): 7.67e-83: 241: 93.20
+	Hs
		957232 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2D3_HUMAN (1-147): 8.57e-82: 238: 91.84
+	Hs
		1397303 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2E2_HUMAN (4-146): 2.83e-75: 224: 64.34
+	Hs
		1381218 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2E3_HUMAN (4-146): 1.06e-74: 223: 64.34
+	Hs
		957760 Q6GX22_HUMAN (1-665): 9.33e-171: 505: 76.24		UBE2N_HUMAN (4-147): 2.31e-69: 207: 46.53
+	Hs
		909409 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UB2E1_HUMAN (4-146): 3.9e-73: 218: 64.34
+	Hs
		957862 TRIM1_HUMAN (1-665): 2.39e-164: 491: 72.81		UBE2N_HUMAN (4-147): 2.31e-69: 207: 46.53
+	Hs
		906666 TRI25_HUMAN (4-656): 1.55e-105: 335: 23.57		UB2D1_HUMAN (1-147): 3.33e-83: 242: 91.84
+	Hs
		957079 TRI25_HUMAN (4-656): 1.55e-105: 335: 23.57		UB2D3_HUMAN (1-147): 8.57e-82: 238: 91.84
+	Hs
		957737 TRI25_HUMAN (4-656): 1.55e-105: 335: 23.57		UBE2N_HUMAN (4-147): 2.31e-69: 207: 46.53