HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: SF3B4 (Q15427)
Splicing factor 3B subunit 4 (Pre-mRNA-splicing factor SF3b 49 kDa subunit) (Spliceosome-associated protein 49) (SAP 49)
Gene: SF3B4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.82	0.50	0.644

Property	Details
Experiments	25416956, 16189514, 32296183, unassigned1304
Identification Methods	two hybrid, validated two hybrid, two hybrid prey pooling approach, two hybrid array, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	-
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 424
+	Hs
		594008 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		SF3B4_HUMAN (1-424): 2.4e-58: 198: 100.00
+	Hs
		1148498 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		SF3B4_HUMAN (1-424): 2.4e-58: 198: 100.00
+	Hs
		440571 FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85		SF3B4_HUMAN (1-424): 2.4e-58: 198: 100.00
+	Ce
		1067199 Q95PW9_CAEEL (1-379): 2.06e-70: 227: 37.63		SF3B4_CAEEL (102-387): 1.69e-12: 70.3: 18.18
+	Hs
		841012 FRK_HUMAN (3-171): 1.38e-17: 86: 15.39		SF3B4_HUMAN (1-424): 2.4e-58: 198: 100.00
+	Hs
		987658 GRB2_HUMAN (3-60): 5.23e-18: 84: 29.31		SF3B4_HUMAN (1-424): 2.4e-58: 198: 100.00
+	Dm
		1061485 GRAP_DROME (204-368): 9.23e-17: 80.1: 30.72		Q9W424_DROME (1-347): 3.21e-51: 177: 62.82
+	Hs
		557280 SRC_HUMAN (6-155): 4.28e-19: 90.6: 17.11		PABP4_HUMAN (4-183): 1.21e-37: 146: 38.12
+	Hs
		550100 SRC_HUMAN (6-155): 4.28e-19: 90.6: 17.11		PABP1_HUMAN (4-191): 5.23e-34: 135: 36.51
+	Hs
		557468 SRC_HUMAN (6-155): 4.28e-19: 90.6: 17.11		PABP3_HUMAN (4-191): 1.01e-32: 132: 33.86
+	Hs
		550216 GRB2_HUMAN (3-60): 5.23e-18: 84: 29.31		PABP1_HUMAN (4-191): 5.23e-34: 135: 36.51
+	Hs
		463106 YES_HUMAN (6-137): 1.81e-19: 91.7: 20.15		ELAV1_HUMAN (101-322): 9.32e-15: 76.9: 17.33
+	Hs
		987626 GRB2_HUMAN (3-60): 5.23e-18: 84: 29.31		HNRPD_HUMAN (87-185): 1.97e-15: 79.2: 25.25
+	Ce
		234040 SRC2_CAEEL (1-194): 3.61e-19: 91: 17.73		SF3B4_CAEEL (102-387): 1.69e-12: 70.3: 18.18
+	Ce
		1067202 Q9U2T9_CAEEL (51-302): 9.43e-15: 78.2: 18.40		SF3B4_CAEEL (102-387): 1.69e-12: 70.3: 18.18
+	Ce
		711453 SEM5_CAEEL (204-364): 4.92e-16: 78.6: 30.68		Q17350_CAEEL (11-183): 5.05e-10: 63.5: 23.96