HitPredict

Interactor 1: PRKN (O60260)
E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2)
Gene: PRKN

Interactor 2: PSB10 (P40306)
Proteasome subunit beta type-10 (EC 3.4.25.1) (Low molecular mass protein 10) (Macropain subunit MECl-1) (Multicatalytic endopeptidase complex subunit MECl-1) (Proteasome MECl-1) (Proteasome subunit beta-2i)
Gene: PSMB10

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.44	0.60	0.515

Property	Details
Experiments	32814053
Identification Methods	two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 465 Hs			1 273
+	Hs
		228452 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		PSB7_HUMAN (7-272): 6.49e-92: 274: 57.84
+	Hs
		226593 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		PSB5_HUMAN (27-240): 2.59e-86: 259: 26.98
+	Hs
		226567 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		PSA4_HUMAN (6-238): 1.01e-84: 255: 21.25
+	Hs
		226590 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		PSA5_HUMAN (8-240): 5.91e-81: 245: 20.58
+	Hs
		226592 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		PSB6_HUMAN (36-225): 4.48e-73: 225: 29.32
+	Hs
		474931 UBB_HUMAN (1-234): 1.14e-59: 197: 17.92		PSA4_HUMAN (6-238): 1.01e-84: 255: 21.25
+	Hs
		476251 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		PSB7_HUMAN (7-272): 6.49e-92: 274: 57.84
+	At
		286456 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		PSA4A_ARATH (6-261): 9.62e-90: 268: 19.08
+	At
		1091724 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		PSB7B_ARATH (6-271): 4.78e-87: 262: 50.37
+	Hs
		475371 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		PSB5_HUMAN (27-240): 2.59e-86: 259: 26.98
+	At
		188702 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		PSB7A_ARATH (6-271): 3.83e-86: 259: 49.44
+	At
		188713 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		PSB5A_ARATH (38-249): 1.06e-82: 250: 28.64
+	At
		1092123 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		PSB5B_ARATH (13-249): 2.84e-82: 249: 26.89
+	At
		188696 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		PSA2A_ARATH (8-238): 4.51e-82: 248: 22.88
+	Hs
		475369 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		PSA5_HUMAN (8-240): 5.91e-81: 245: 20.58
+	At
		1091773 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		PSA2B_ARATH (8-238): 4.04e-81: 245: 23.31
+	Hs
		475370 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		PSB6_HUMAN (36-225): 4.48e-73: 225: 29.32
+	Zm
		35351 Q42415_MAIZE (1-153): 8.84e-50: 179: 20.13		A0A1D6LKR2_MAIZE (37-223): 6.58e-22: 93.2: 29.69
+	At
		1091802 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		PSB6_ARATH (30-221): 6.11e-26: 103: 32.12
+	Zm
		35350 Q41754_MAIZE (1-76): 8.11e-30: 121: 31.58		A0A1D6LKR2_MAIZE (37-223): 6.58e-22: 93.2: 29.69