HitPredict

Interactor 1: RPB1 (P04050)
DNA-directed RNA polymerase II subunit RPB1 (RNA polymerase II subunit 1) (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit) (RNA polymerase II subunit B220)
Gene: RPO21

Interactor 2: TFS2 (P07273)
Transcription elongation factor S-II (DNA strand transfer protein alpha) (STP-alpha) (DNA strand transferase 1) (Pyrimidine pathway regulatory protein 2)
Gene: DST1

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.967

Property	Details
Experiments	9573141, 9450930, 12242279, 16554755, 25776559, 28585565, 33226341, 25029256
Identification Methods	affinity chromatography technology, pull down, two hybrid, tandem affinity purification
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	RNA_pol_Rpb1_2~TFIIS_C, RNA_pol_Rpb1_3~TFIIS_C, RNA_pol_Rpb1_4~TFIIS_C, RNA_pol_Rpb1_4~TFIIS_M, RNA_pol_Rpb1_5~TFIIS_C, RNA_pol_Rpb1_5~TFIIS_M
Common GO terms	GO:0005634 - nucleus GO:0006367 - transcription initiation at RNA polymerase II promoter GO:0006368 - transcription elongation by RNA polymerase II
Homologous interactions	7

Homologous interactions

Color coding for hit scores

	1 1733 Sc			1 309
+	Dm
		375672 RPB1_DROME (1-1731): 0: 1752: 46.83		Q9VXS6_DROME (141-307): 9.26e-60: 190: 29.34
+	Sc
		375361 RPB1_YEAST (1-1555): 0: 1770: 100.00		BYE1_YEAST (65-293): 3.7e-39: 146: 13.25
+	Sc
		375628 RPC1_YEAST (1-1448): 0: 1370: 31.72		RPC10_YEAST (245-307): 2.15e-23: 94.1: 31.82
+	Hs
		156085 RPC1_HUMAN (1-1457): 0: 1366: 31.97		RPC10_HUMAN (241-307): 1.09e-22: 92.2: 34.72
+	Hs
		612955 RPB1_HUMAN (1529-1719): 2.52e-19: 97.6: 70.23		TCEA1_HUMAN (3-308): 1.07e-97: 291: 29.45
+	Mm
		444976 RPB1_MOUSE (1518-1732): 2.59e-14: 81.1: 65.53		DIDO1_MOUSE (44-254): 7.91e-47: 170: 16.51
+	Hs
		317191 RPA1_HUMAN (912-1446): 2.52e-35: 150: 14.81		RPC10_HUMAN (241-307): 1.09e-22: 92.2: 34.72