HitPredict

Interactor 1: RB (P06400)
Retinoblastoma-associated protein (p105-Rb) (p110-RB1) (pRb) (Rb) (pp110)
Gene: RB1

Interactor 2: E2F5 (Q15329)
Transcription factor E2F5 (E2F-5)
Gene: E2F5

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.69	1.00	0.834

Property	Details
Experiments	24981860, 12502741, 16360038
Identification Methods	affinity chromatography technology, isothermal titration calorimetry, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	E2F_CC-MB~Rb_C
Common GO terms	GO:0000785 - chromatin GO:0005654 - nucleoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 928 Hs			1 346
+	Hs
		414284 RB_HUMAN (1-928): 0: 1323: 100.00		E2F2_HUMAN (10-340): 1.51e-101: 307: 35.00
+	Hs
		135199 RB_HUMAN (1-928): 0: 1323: 100.00		E2F3_HUMAN (8-340): 3.77e-100: 305: 32.64
+	Hs
		414250 RB_HUMAN (1-928): 0: 1323: 100.00		E2F1_HUMAN (10-340): 1.7e-95: 292: 29.54
+	Mm
		192503 RB_MOUSE (24-928): 0: 1260: 91.38		E2F3_MOUSE (8-340): 2.02e-100: 305: 32.64
+	Rn
		140344 RB_RAT (1-928): 0: 1274: 88.90		E2F1_RAT (32-340): 1.79e-93: 287: 33.74
+	Mm
		527140 RB_MOUSE (24-928): 0: 1260: 91.38		E2F2_MOUSE (10-340): 2.42e-96: 294: 33.79
+	Mm
		527156 RB_MOUSE (24-928): 0: 1260: 91.38		E2F1_MOUSE (32-340): 1.02e-93: 287: 34.25
+	Hs
		265585 RB_HUMAN (1-928): 0: 1323: 100.00		E2F6_HUMAN (50-258): 9.84e-70: 220: 36.02
+	Hs
		414299 RB_HUMAN (1-928): 0: 1323: 100.00		E2F4_HUMAN (268-345): 2.71e-13: 72.2: 43.37
+	Mm
		527164 RB_MOUSE (24-928): 0: 1260: 91.38		E2F4_MOUSE (268-344): 1.61e-12: 69.9: 42.68
+	At
		1324895 RBR1_ARATH (19-886): 0: 632: 22.98		E2FA_ARATH (6-339): 9.56e-101: 307: 33.72
+	Dm
		284513 RBF_DROME (26-860): 0: 684: 21.83		E2F2_DROME (2-342): 1.35e-79: 249: 29.70
+	At
		1325150 RBR1_ARATH (19-886): 0: 632: 22.98		E2FB_ARATH (5-339): 2.04e-97: 298: 33.14
+	Dm
		1097882 RBF_DROME (26-860): 0: 684: 21.83		E2F1_DROME (2-269): 1.61e-57: 201: 32.86
+	At
		1325146 RBR1_ARATH (19-886): 0: 632: 22.98		E2FC_ARATH (49-260): 5.35e-73: 233: 40.65
+	Dm
		284545 Q9VF04_DROME (51-764): 1.26e-133: 421: 19.00		E2F2_DROME (2-342): 1.35e-79: 249: 29.70
+	Dm
		284539 Q9NIS1_DROME (51-764): 2.83e-133: 420: 19.00		E2F2_DROME (2-342): 1.35e-79: 249: 29.70
+	Ce
		106296 LIN35_CAEEL (57-783): 2.49e-125: 404: 19.93		G5EF11_CAEEL (1-292): 1.66e-76: 240: 29.09
+	Hs
		135214 RBL1_HUMAN (598-875): 2.06e-76: 272: 28.92		E2F3_HUMAN (8-340): 3.77e-100: 305: 32.64
+	Mm
		1145491 RBL1_MOUSE (594-879): 2.53e-78: 278: 29.52		E2F4_MOUSE (268-344): 1.61e-12: 69.9: 42.68