HitPredict

Interactor 1: ISCS (P0A6B7)
Cysteine desulfurase IscS (EC 2.8.1.7) (NifS protein homolog) (ThiI transpersulfidase) (TusA transpersulfidase)
Gene: ISCS

Interactor 2: TUSA (P0A890)
Sulfur carrier protein TusA (Sulfur mediator TusA) (Sulfur transfer protein TusA) (tRNA 2-thiouridine synthesizing protein A)
Gene: TUSA

Species: Escherichia coli K-12

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.81	1.00	0.903

Property	Details
Experiments	16606699, 24561554, 15690043, 16387657
Identification Methods	affinity chromatography technology, two hybrid, tandem affinity purification, pull down, molecular sieving, two hybrid array
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Aminotran_5~TusA
Common GO terms	GO:0002143 - tRNA wobble position uridine thiolation GO:0005829 - cytosol GO:0019448 - L-cysteine catabolic process GO:0097163 - sulfur carrier activity GO:1990329 - IscS-TusA complex
Homologous interactions	1

Homologous interactions

Color coding for hit scores

	1 404 Ec			1 81
+	Ec
		457420 ISCS_ECO57 (1-404): 2.4e-138: 402: 100.00		TUSA_ECO57 (1-81): 8.03e-56: 168: 100.00