HitPredict

Interactor 1: UCHL1 (P09936)
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) (EC 3.4.19.12) (Neuron cytoplasmic protein 9.5) (PGP 9.5) (PGP9.5) (Ubiquitin thioesterase L1)
Gene: UCHL1

Interactor 2: NEDD8 (Q15843)
Ubiquitin-like protein NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8)
Gene: NEDD8

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	1.00	0.811

Property	Details
Experiments	16169070, 32814053
Identification Methods	two hybrid, two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Peptidase_C12~ubiquitin
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 223 Hs			1 81
+	Mm
		714576 UCHL3_MOUSE (2-222): 2.67e-101: 294: 55.31		NEDD8_MOUSE (1-81): 1.25e-41: 132: 98.77
+	Hs
		488443 UCHL1_HUMAN (1-223): 2.39e-103: 299: 100.00		RS27A_HUMAN (1-77): 6.68e-37: 122: 57.14
+	Hs
		488379 UCHL1_HUMAN (1-223): 2.39e-103: 299: 100.00		UBB_HUMAN (1-76): 2.41e-30: 107: 57.90
+	Hs
		507066 UCHL3_HUMAN (2-222): 3.84e-101: 294: 54.43		UBB_HUMAN (1-76): 2.41e-30: 107: 57.90
+	Hs
		583873 UCHL3_HUMAN (2-222): 3.84e-101: 294: 54.43		Q5U5U6_HUMAN (1-76): 2.41e-30: 107: 57.90
+	Hs
		583868 UCHL3_HUMAN (2-222): 3.84e-101: 294: 54.43		NEDD8_HUMAN (1-81): 2.08e-30: 103: 100.00
+	Dm
		583729 Q9XZ61_DROME (2-220): 1.38e-90: 271: 22.03		RS27A_DROME (1-77): 3.4e-36: 121: 57.14
+	Hs
		987140 BAP1_HUMAN (3-220): 6.72e-78: 249: 22.27		RS27A_HUMAN (1-77): 6.68e-37: 122: 57.14
+	Dm
		583681 CALYP_DROME (3-220): 1.73e-76: 239: 22.69		RS27A_DROME (1-77): 3.4e-36: 121: 57.14
+	Sp
		154031 UBLH2_SCHPO (1-221): 2.77e-85: 256: 22.71		UBL1_SCHPO (1-76): 9.68e-30: 102: 81.58
+	Sp
		154028 UBLH1_SCHPO (3-220): 2.3e-68: 210: 32.30		UBL1_SCHPO (1-76): 9.68e-30: 102: 81.58
+	Sc
		509887 UBL1_YEAST (5-219): 6.44e-54: 174: 26.29		RL40A_YEAST (1-76): 1.88e-32: 110: 55.26
+	Sc
		436370 UBL1_YEAST (5-219): 6.44e-54: 174: 26.29		RS31_YEAST (1-70): 3.05e-30: 105: 52.86
+	Sc
		762712 UBL1_YEAST (5-219): 6.44e-54: 174: 26.29		RUB1_YEAST (1-76): 1.46e-21: 81.3: 59.21