HitPredict

Interactor 1: VPS4 (P52917)
Vacuolar protein sorting-associated protein 4 (DOA4-independent degradation protein 6) (Protein END13) (Vacuolar protein-targeting protein 10)
Gene: VPS4

Interactor 2: DID2 (P69771)
Vacuolar protein-sorting-associated protein 46 (Charged multivesicular body protein 1) (DOA4-independent degradation protein 2) (Fifty two inhibitor 1)
Gene: DID2

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	0.60	0.759

Property	Details
Experiments	17928861, 16601096, 24711499, 15086794, 16704411, 18266866, 17130288, 17408385, 37968396, 20110351, 18194652, 22118530
Identification Methods	classical fluorescence spectroscopy, pull down, affinity chromatography technology, two hybrid, far western blotting
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0015031 - protein transport GO:0032511 - late endosome to vacuole transport via multivesicular body sorting pathway GO:0045324 - late endosome to vacuole transport
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 437 Sc			1 204
+	Sc
		732854 VPS4_YEAST (1-437): 9.44e-127: 375: 100.00		DID4_YEAST (4-203): 4.54e-43: 146: 18.89
+	At
		1352475 VPS4_ARATH (4-436): 3.27e-116: 348: 53.46		VPS2A_ARATH (3-203): 5.95e-47: 155: 21.03
+	Sc
		732543 VPS4_YEAST (1-437): 9.44e-127: 375: 100.00		VPS24_YEAST (4-203): 1.13e-34: 124: 16.51
+	Hs
		1357379 VPS4A_HUMAN (3-435): 7.08e-117: 350: 58.73		CHMP3_HUMAN (3-204): 2.43e-43: 146: 13.15
+	Hs
		260164 VPS4B_HUMAN (2-436): 2.84e-118: 354: 60.50		CHM2B_HUMAN (3-202): 1.2e-41: 141: 14.29
+	Hs
		208804 VPS4B_HUMAN (2-436): 2.84e-118: 354: 60.50		CHM2A_HUMAN (3-181): 5.76e-41: 140: 21.67
+	At
		1352776 VPS4_ARATH (4-436): 3.27e-116: 348: 53.46		CHM1B_ARATH (5-204): 1.8e-42: 143: 36.82
+	Hs
		1357378 VPS4A_HUMAN (3-435): 7.08e-117: 350: 58.73		CHM2B_HUMAN (3-202): 1.2e-41: 141: 14.29
+	At
		1013169 VPS4_ARATH (4-436): 3.27e-116: 348: 53.46		VPS2B_ARATH (4-203): 6.03e-42: 142: 17.45
+	Hs
		208869 VPS4A_HUMAN (3-435): 7.08e-117: 350: 58.73		CHM2A_HUMAN (3-181): 5.76e-41: 140: 21.67
+	Mm
		804060 VPS4B_MOUSE (2-436): 2.92e-117: 351: 60.72		CHM2A_MOUSE (3-192): 9.37e-41: 139: 20.94
+	Dm
		1373132 Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43		Q9VRJ5_DROME (4-204): 6.57e-43: 144: 17.33
+	Hs
		260156 VPS4B_HUMAN (2-436): 2.84e-118: 354: 60.50		CHM1A_HUMAN (9-203): 1.04e-38: 133: 45.92
+	Dm
		1371193 Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43		Q9VN02_DROME (2-204): 3.38e-42: 143: 13.49
+	Hs
		1336687 VPS4A_HUMAN (3-435): 7.08e-117: 350: 58.73		CHM1A_HUMAN (9-203): 1.04e-38: 133: 45.92
+	Hs
		260145 VPS4B_HUMAN (2-436): 2.84e-118: 354: 60.50		CHM1B_HUMAN (7-203): 6.17e-37: 129: 41.41
+	Hs
		1185556 VPS4A_HUMAN (3-435): 7.08e-117: 350: 58.73		CHM1B_HUMAN (7-203): 6.17e-37: 129: 41.41
+	Dm
		1279493 Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43		Q95SH2_DROME (5-203): 3.25e-39: 135: 42.50
+	Dm
		1362012 Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43		Q9VBI3_DROME (4-194): 2.98e-38: 134: 19.79
+	Dm
		1248702 Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43		Q8SXB1_DROME (8-194): 1.66e-34: 124: 20.21