HitPredict

Interactor 1: RHOB (P62745)
Rho-related GTP-binding protein RhoB (Rho cDNA clone 6) (h6)
Gene: RHOB

Interactor 2: SMUF1 (Q9HCE7)
E3 ubiquitin-protein ligase SMURF1 (hSMURF1) (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase SMURF1) (SMAD ubiquitination regulatory factor 1) (SMAD-specific E3 ubiquitin-protein ligase 1)
Gene: SMURF1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.68	0.60	0.639

Property	Details
Experiments	30587574, 25249323
Identification Methods	enzymatic study, anti bait coimmunoprecipitation, pull down, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0030154 - cell differentiation GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 196 Hs			1 757
+	Hs
		900929 RHOA_HUMAN (1-196): 7.19e-78: 232: 83.67		SMUF1_HUMAN (1-757): 0: 891: 100.00
+	Rn
		88544 RHOA_RAT (1-196): 3.27e-78: 233: 83.16		D3ZCF5_RAT (1-757): 0: 825: 93.53
+	Mm
		56825 RAC1_MOUSE (1-196): 2.37e-79: 236: 55.10		SMUF2_MOUSE (1-757): 0: 795: 71.30
+	Hs
		900923 RHOA_HUMAN (1-196): 7.19e-78: 232: 83.67		SMUF2_HUMAN (1-757): 0: 794: 70.72
+	Hs
		925520 RAC1_HUMAN (1-196): 2.37e-79: 236: 55.10		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		900802 RHOA_HUMAN (1-196): 7.19e-78: 232: 83.67		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		915895 RHOB_HUMAN (1-196): 7.52e-78: 232: 100.00		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		545560 RAC2_HUMAN (1-196): 7.63e-77: 230: 53.57		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		436457 RHOC_HUMAN (1-196): 8.84e-76: 227: 83.16		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		951237 RHOG_HUMAN (1-196): 8.84e-76: 227: 51.53		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		125349 RAC1_HUMAN (1-196): 2.37e-79: 236: 55.10		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125308 RHOA_HUMAN (1-196): 7.19e-78: 232: 83.67		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125330 RHOB_HUMAN (1-196): 7.52e-78: 232: 100.00		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125135 RAC2_HUMAN (1-196): 7.63e-77: 230: 53.57		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125100 RHOC_HUMAN (1-196): 8.84e-76: 227: 83.16		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125372 RHOG_HUMAN (1-196): 8.84e-76: 227: 51.53		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125298 CDC42_HUMAN (1-196): 1.35e-74: 224: 49.49		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		808080 RAC1_HUMAN (1-196): 2.37e-79: 236: 55.10		NEDD4_HUMAN (93-755): 0: 673: 36.63
+	Hs
		925397 RAC1_HUMAN (1-196): 2.37e-79: 236: 55.10		HUWE1_HUMAN (71-756): 0: 583: 30.14
+	Hs
		915900 RHOB_HUMAN (1-196): 7.52e-78: 232: 100.00		NED4L_HUMAN (6-396): 7.88e-36: 147: 16.88