HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: SH24A (Q9H788)
SH2 domain-containing protein 4A (Protein SH(2)A) (Protein phosphatase 1 regulatory subunit 38)
Gene: SH2D4A

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	32296183, 20936779, unassigned1304
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	SH2~SH2, SH2~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 454
+	Mm
		1141166 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		SH2B2_MOUSE (141-443): 1.84e-52: 188: 17.36
+	Mm
		729751 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		BTK_MOUSE (137-445): 3.74e-52: 187: 11.85
+	Hs
		145977 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SH2B2_HUMAN (141-444): 7.08e-52: 186: 17.63
+	Hs
		924503 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		BTK_HUMAN (137-445): 1.56e-51: 186: 12.16
+	Hs
		924594 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SHB_HUMAN (206-444): 1.09e-46: 170: 16.48
+	Hs
		924890 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SH2B1_HUMAN (141-450): 3.73e-43: 163: 15.18
+	Hs
		522887 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SRC_HUMAN (238-454): 4.15e-42: 158: 14.56
+	Hs
		788982 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		TEC_HUMAN (203-445): 1.81e-40: 155: 12.74
+	Hs
		924447 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		GRB2_HUMAN (310-454): 1.67e-42: 151: 24.83
+	Hs
		562638 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		PLCG2_HUMAN (98-454): 9.6e-30: 125: 15.11
+	Mm
		1141132 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		PLCG1_MOUSE (90-454): 5.82e-26: 113: 15.25
+	Rn
		499159 GRB2_RAT (1-217): 6.71e-76: 229: 100.00		PLCG1_RAT (90-454): 3.67e-26: 114: 15.25
+	Hs
		580972 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		PLCG1_HUMAN (90-454): 1.69e-25: 112: 14.47
+	Hs
		129269 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		P85B_HUMAN (134-447): 1.04e-20: 97.3: 16.97
+	Mm
		643091 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		P85A_MOUSE (342-451): 3.85e-18: 89.2: 23.48
+	Hs
		655306 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		P85A_HUMAN (341-451): 2.42e-18: 90: 24.14
+	Hs
		924735 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		P55G_HUMAN (341-451): 1.55e-18: 89.6: 21.55
+	Hs
		796538 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		KSYK_HUMAN (273-440): 2.84e-14: 76.8: 23.08
+	Hs
		557655 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		NCK1_HUMAN (346-428): 3.27e-14: 75.7: 30.95
+	Hs
		596390 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		RASA1_HUMAN (347-453): 2.85e-13: 74.1: 25.44