HitPredict

Interactor 1: KMT2A (Q03164)
Histone-lysine N-methyltransferase 2A (Lysine N-methyltransferase 2A) (EC 2.1.1.364) (ALL-1) (CXXC-type zinc finger protein 7) (Cysteine methyltransferase KMT2A) (EC 2.1.1.-) (Myeloid/lymphoid or mixed-lineage leukemia) (Myeloid/lymphoid or mixed-lineage leukemia protein 1) (Trithorax-like protein) (Zinc finger protein HRX) [Cleaved into: MLL cleavage product N320 (N-terminal cleavage product of 320 kDa) (p320); MLL cleavage product C180 (C-terminal cleavage product of 180 kDa) (p180)]
Gene: KMT2A

Interactor 2: AFF4 (Q9UHB7)
AF4/FMR2 family member 4 (ALL1-fused gene from chromosome 5q31 protein) (Protein AF-5q31) (Major CDK9 elongation factor-associated protein)
Gene: AFF4

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.62	0.60	0.611

Property	Details
Experiments	20153263, 22094252
Identification Methods	affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005654 - nucleoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 3969 Hs			1 1163
+	Hs
		850807 KMT2A_HUMAN (1-3969): 0: 3187: 100.00		AFF1_HUMAN (4-1160): 4.79e-88: 312: 34.47
+	Hs
		975290 KMT2A_HUMAN (1-3969): 0: 3187: 100.00		Q8TD98_HUMAN (305-773): 2.44e-30: 130: 32.92
+	Hs
		975285 KMT2A_HUMAN (1-3969): 0: 3187: 100.00		KMT2C_HUMAN (263-920): 1.07e-09: 65.4: 9.56
+	Hs
		850820 Q8TD98_HUMAN (1311-1447): 2.49e-58: 217: 98.54		AFF1_HUMAN (4-1160): 4.79e-88: 312: 34.47
+	Hs
		1243852 KMT2B_HUMAN (3163-3969): 3.56e-127: 454: 36.71		KMT2C_HUMAN (263-920): 1.07e-09: 65.4: 9.56
+	Hs
		1246387 KMT2B_HUMAN (3163-3969): 3.56e-127: 454: 36.71		THOC2_HUMAN (501-879): 3.93e-09: 63.5: 14.63
+	Mm
		1142273 EZH2_MOUSE (3450-3951): 3.64e-105: 358: 18.54		ATRX_MOUSE (45-862): 1.63e-31: 136: 12.95
+	Hs
		1080420 EZH2_HUMAN (3450-3951): 9.4e-105: 357: 18.54		CHD7_HUMAN (73-891): 6.5e-25: 115: 12.85
+	Hs
		1080263 EZH2_HUMAN (3450-3951): 9.4e-105: 357: 18.54		MUC4_HUMAN (26-1086): 8.48e-24: 111: 9.92
+	Hs
		1080112 EZH2_HUMAN (3450-3951): 9.4e-105: 357: 18.54		CRBG2_HUMAN (420-1083): 9.55e-16: 85.1: 9.85
+	Hs
		1056971 SETD2_HUMAN (3398-3968): 1.14e-75: 285: 16.61		MDC1_HUMAN (67-908): 1.82e-35: 149: 9.85
+	Hs
		1080127 EZH2_HUMAN (3450-3951): 9.4e-105: 357: 18.54		KMT2C_HUMAN (263-920): 1.07e-09: 65.4: 9.56
+	Hs
		331812 NSD2_HUMAN (3752-3968): 7.27e-60: 231: 27.23		MDC1_HUMAN (67-908): 1.82e-35: 149: 9.85
+	Dm
		1129083 SET1_DROME (3423-3969): 3.44e-81: 301: 21.22		Q9VLS7_DROME (52-1070): 6.32e-13: 75.9: 7.69
+	Hs
		1243853 SET1B_HUMAN (3529-3969): 1.64e-66: 254: 27.12		KMT2C_HUMAN (263-920): 1.07e-09: 65.4: 9.56
+	Hs
		1231748 MINT_HUMAN (418-1102): 7.18e-09: 64.7: 11.18		AN36B_HUMAN (555-1125): 1.21e-59: 226: 10.33
+	Hs
		165699 SET1A_HUMAN (3695-3969): 8.18e-56: 218: 34.58		KMT2C_HUMAN (263-920): 1.07e-09: 65.4: 9.56
+	Dm
		65570 FUTSC_DROME (736-1358): 3.26e-10: 69.1: 12.74		A8JNM5_DROME (60-869): 2.36e-28: 126: 9.18
+	Mm
		1351630 PCLO_MOUSE (755-1426): 4.31e-12: 75.3: 12.23		PCLO_MOUSE (7-987): 3.87e-21: 102: 8.11
+	Hs
		152667 KMT2D_HUMAN (11-1393): 8.17e-09: 64.4: 10.47		KMT2C_HUMAN (263-920): 1.07e-09: 65.4: 9.56