HitPredict

Interactor 1: RUB1 (Q03919)
NEDD8-like protein RUB1 (Related to ubiquitin protein 1) (Ubiquitin-like protein RUB1)
Gene: RUB1

Interactor 2: CDC53 (Q12018)
Cell division control protein 53 (Cullin-A) (E3 ubiquitin ligase complex SCF subunit CDC53)
Gene: CDC53

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.860

Property	Details
Experiments	9531531, 14519104, 19882662, 19942853
Identification Methods	coimmunoprecipitation, comigration in sds page, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Cullin~ubiquitin, Cullin_Nedd8~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 77 Sc			1 815
+	Hs
		1041004 NEDD8_HUMAN (1-76): 1.61e-34: 113: 59.21		CUL1_HUMAN (7-815): 0: 741: 29.92
+	Hs
		923984 RS27A_HUMAN (1-76): 3.48e-37: 123: 52.63		CUL4A_HUMAN (6-815): 0: 701: 21.29
+	Dm
		1097203 NEDD8_DROME (1-77): 2.58e-33: 111: 59.74		CUL1_DROME (2-815): 0: 708: 28.25
+	Hs
		923983 RS27A_HUMAN (1-76): 3.48e-37: 123: 52.63		CUL3_HUMAN (10-815): 0: 696: 21.12
+	Hs
		923985 RS27A_HUMAN (1-76): 3.48e-37: 123: 52.63		CUL4B_HUMAN (5-815): 0: 695: 21.14
+	Hs
		924296 RL40_HUMAN (1-76): 2.25e-34: 115: 52.63		CUL4A_HUMAN (6-815): 0: 701: 21.29
+	Hs
		1043010 NEDD8_HUMAN (1-76): 1.61e-34: 113: 59.21		CUL4A_HUMAN (6-815): 0: 701: 21.29
+	At
		1352191 RUB1_ARATH (3-76): 5.75e-27: 97.1: 58.11		CUL3A_ARATH (5-815): 0: 714: 22.49
+	Hs
		1041658 NEDD8_HUMAN (1-76): 1.61e-34: 113: 59.21		CUL3_HUMAN (10-815): 0: 696: 21.12
+	Hs
		1043371 NEDD8_HUMAN (1-76): 1.61e-34: 113: 59.21		CUL4B_HUMAN (5-815): 0: 695: 21.14
+	Sp
		142907 UBL1_SCHPO (1-77): 1.91e-33: 111: 59.74		CUL1_SCHPO (7-815): 0: 697: 31.37
+	Hs
		475024 UBB_HUMAN (1-76): 2.37e-31: 110: 52.63		CUL4B_HUMAN (5-815): 0: 695: 21.14
+	At
		1321116 RUB1_ARATH (3-76): 5.75e-27: 97.1: 58.11		CUL3B_ARATH (5-815): 0: 704: 22.37
+	Dm
		1213846 NEDD8_DROME (1-77): 2.58e-33: 111: 59.74		Q8IP45_DROME (2-815): 0: 678: 21.37
+	Hs
		1041337 NEDD8_HUMAN (1-76): 1.61e-34: 113: 59.21		CUL2_HUMAN (3-815): 0: 671: 26.08
+	At
		1277343 RUB1_ARATH (3-76): 5.75e-27: 97.1: 58.11		CUL1_ARATH (4-815): 0: 678: 21.28
+	At
		1247030 RUB2_ARATH (3-76): 6.74e-26: 94.4: 58.11		CUL1_ARATH (4-815): 0: 678: 21.28
+	Ce
		1088883 NEDD8_CAEEL (1-76): 1.03e-32: 109: 60.53		CUL3_CAEEL (1-815): 0: 616: 20.91
+	Hs
		1079566 NEDD8_HUMAN (1-76): 1.61e-34: 113: 59.21		CUL5_HUMAN (8-815): 0: 590: 20.84
+	Sp
		145590 UBL1_SCHPO (1-77): 1.91e-33: 111: 59.74		CUL3_SCHPO (7-815): 0: 576: 19.52