HitPredict

Interactor 1: EP300 (Q09472)
Histone acetyltransferase p300 (p300 HAT) (EC 2.3.1.48) (E1A-associated protein p300) (Histone butyryltransferase p300) (EC 2.3.1.-) (Histone crotonyltransferase p300) (EC 2.3.1.-) (Protein 2-hydroxyisobutyryltransferase p300) (EC 2.3.1.-) (Protein lactyltransferas p300) (EC 2.3.1.-) (Protein propionyltransferase p300) (EC 2.3.1.-)
Gene: EP300

Interactor 2: M3K5 (Q99683)
Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.11.25) (Apoptosis signal-regulating kinase 1) (ASK-1) (MAPK/ERK kinase kinase 5) (MEK kinase 5) (MEKK 5)
Gene: MAP3K5

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	0.60	0.541

Property	Details
Experiments	20936779
Identification Methods	two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0045893 - positive regulation of DNA-templated transcription
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 2414 Hs			1 1374
+	Hs
		1067110 BRD3_HUMAN (1055-1159): 9.06e-30: 130: 30.48		M3K5_HUMAN (1-1374): 0: 1497: 100.00
+	Hs
		1270773 CBP_HUMAN (1752-2400): 4.44e-10: 67.9: 13.25		M3K5_HUMAN (1-1374): 0: 1497: 100.00
+	Hs
		254474 EP300_HUMAN (1-2414): 0: 1113: 100.00		ROCK2_HUMAN (668-1343): 7.3e-114: 392: 17.55
+	Hs
		270406 EP300_HUMAN (1-2414): 0: 1113: 100.00		KS6A5_HUMAN (673-1088): 5.06e-109: 365: 19.51
+	Hs
		998640 EP300_HUMAN (1-2414): 0: 1113: 100.00		PAK2_HUMAN (686-960): 1.26e-97: 325: 31.27
+	Hs
		681812 EP300_HUMAN (1-2414): 0: 1113: 100.00		AKT1_HUMAN (438-615): 7.18e-25: 112: 13.64
+	Hs
		1356278 TRI33_HUMAN (604-1201): 1.37e-44: 180: 14.50		KS6A6_HUMAN (673-1074): 2.65e-113: 375: 21.31
+	Hs
		1183084 CBP_HUMAN (1752-2400): 4.44e-10: 67.9: 13.25		MARK2_HUMAN (629-1374): 9.11e-149: 472: 14.03
+	Hs
		850689 CBP_HUMAN (1752-2400): 4.44e-10: 67.9: 13.25		KS6A3_HUMAN (623-1074): 1.34e-117: 387: 19.83
+	Hs
		851493 MDC1_HUMAN (455-1061): 8.69e-13: 76.6: 11.82		NEK4_HUMAN (680-1303): 2.63e-113: 378: 19.70
+	Mm
		101422 F8VPR5_MOUSE (1752-2401): 7.8e-10: 67.1: 12.31		KS6A3_MOUSE (623-1074): 9.86e-118: 387: 19.83
+	Hs
		1071667 CBP_HUMAN (1752-2400): 4.44e-10: 67.9: 13.25		KS6A2_HUMAN (673-1074): 1.52e-117: 386: 20.91
+	Hs
		1057021 MDC1_HUMAN (455-1061): 8.69e-13: 76.6: 11.82		KS6A6_HUMAN (673-1074): 2.65e-113: 375: 21.31
+	Hs
		160565 MDC1_HUMAN (455-1061): 8.69e-13: 76.6: 11.82		AURKA_HUMAN (551-947): 1.43e-115: 369: 22.36
+	Hs
		1073459 CBP_HUMAN (1752-2400): 4.44e-10: 67.9: 13.25		KS6A1_HUMAN (629-1074): 4.77e-113: 374: 18.70
+	Hs
		270431 CBP_HUMAN (1752-2400): 4.44e-10: 67.9: 13.25		KS6A5_HUMAN (673-1088): 5.06e-109: 365: 19.51
+	Sc
		964736 BDF2_YEAST (1072-1149): 1.81e-11: 71.6: 44.87		BCK1_YEAST (685-969): 7.75e-43: 173: 38.59
+	Hs
		681959 CBP_HUMAN (1752-2400): 4.44e-10: 67.9: 13.25		AKT1_HUMAN (438-615): 7.18e-25: 112: 13.64