HitPredict

Interactor 1: BUB3 (O43684)
Mitotic checkpoint protein BUB3
Gene: BUB3

Interactor 2: CDC20 (Q12834)
Cell division cycle protein 20 homolog (p55CDC)
Gene: CDC20

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.979

Property	Details
Experiments	24464857, 25833949, 25482201, 25306918, 26811472, 27114510, 28514442, 28410192, 33961781, 35913887, 21926987, 23643811, 23443559, 21407176
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	ANAPC4_WD40~WD40
Common GO terms	GO:0000776 - kinetochore GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0007094 - mitotic spindle assembly checkpoint signaling GO:0033597 - mitotic checkpoint complex and 1 others more... GO:0051301 - cell division
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 328 Hs			1 499
+	Hs
		211260 BUB3_HUMAN (1-328): 4.65e-94: 284: 100.00		NLE1_HUMAN (64-471): 2.26e-89: 283: 18.93
+	Hs
		943879 RAE1L_HUMAN (3-326): 2.75e-99: 299: 33.63		FBXW7_HUMAN (210-496): 2.2e-71: 242: 17.81
+	Sp
		999842 TUP11_SCHPO (1-213): 6.22e-64: 214: 17.90		TUP12_SCHPO (46-475): 1.94e-91: 292: 19.87
+	Hs
		1346954 NLE1_HUMAN (86-326): 2.9e-49: 172: 13.10		NLE1_HUMAN (64-471): 2.26e-89: 283: 18.93
+	Sp
		999838 TUP11_SCHPO (1-213): 6.22e-64: 214: 17.90		TUP11_SCHPO (6-497): 3.96e-57: 201: 15.46
+	Hs
		1281292 FBXW7_HUMAN (1-202): 6.93e-47: 169: 20.85		FBXW7_HUMAN (210-496): 2.2e-71: 242: 17.81
+	Hs
		211280 BUB3_HUMAN (1-328): 4.65e-94: 284: 100.00		FZR1_HUMAN (315-495): 1.51e-24: 108: 19.02
+	Hs
		211150 BUB3_HUMAN (1-328): 4.65e-94: 284: 100.00		WDR5_HUMAN (55-254): 8.3e-18: 86.5: 9.66
+	At
		1338310 BUB31_ARATH (1-327): 1.27e-85: 262: 51.80		CDC21_ARATH (378-499): 4.89e-16: 82.3: 17.32
+	At
		1338308 BUB31_ARATH (1-327): 1.27e-85: 262: 51.80		CDC22_ARATH (378-499): 5.64e-16: 81.9: 17.32
+	Sc
		640829 BUB3_YEAST (7-298): 2.45e-77: 241: 29.78		CDC20_YEAST (315-495): 5.18e-14: 76.6: 18.13
+	Mm
		925836 LIS1_MOUSE (168-319): 3.02e-31: 123: 14.47		LIS1_MOUSE (337-498): 1.47e-37: 144: 16.56
+	Hs
		792935 LIS1_HUMAN (168-319): 3.74e-31: 122: 14.47		LIS1_HUMAN (337-498): 1.74e-37: 143: 16.56
+	Hs
		903551 NLE1_HUMAN (86-326): 2.9e-49: 172: 13.10		WDR5_HUMAN (55-254): 8.3e-18: 86.5: 9.66
+	Hs
		903418 FBXW7_HUMAN (1-202): 6.93e-47: 169: 20.85		WDR5_HUMAN (55-254): 8.3e-18: 86.5: 9.66
+	Hs
		1240442 POC1B_HUMAN (134-327): 7.29e-41: 150: 13.93		POC1A_HUMAN (434-498): 5.08e-13: 72.7: 21.54
+	Hs
		792949 LIS1_HUMAN (168-319): 3.74e-31: 122: 14.47		WDR5_HUMAN (55-254): 8.3e-18: 86.5: 9.66
+	Hs
		793026 LIS1_HUMAN (168-319): 3.74e-31: 122: 14.47		KTNB1_HUMAN (321-470): 1.17e-09: 62.8: 30.26
+	Hs
		1074780 TAF5_HUMAN (65-295): 2.05e-24: 105: 18.35		TAF5_HUMAN (239-336): 9.1e-10: 63.2: 37.00