HitPredict

Interactor 1: RASN (P01111)
GTPase NRas (EC 3.6.5.2) (Transforming protein N-Ras)
Gene: NRAS

Interactor 2: Q96II5 (Q96II5)
non-specific serine/threonine protein kinase (EC 2.7.11.1)
Gene: ARAF

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.44	0.80	0.594

Property	Details
Experiments	unassigned1304
Identification Methods	two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	C1_1~Ras, PK_Tyr_Ser-Thr~Ras, RBD~Ras
Common GO terms	-
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 189 Hs			1 609
+	Hs
		375839 RASK_HUMAN (16-189): 7.11e-85: 249: 85.71		Q96II5_HUMAN (1-609): 0: 603: 100.00
+	Hs
		375113 RASK_HUMAN (16-189): 7.11e-85: 249: 85.71		ARAF_HUMAN (1-609): 0: 599: 99.34
+	Hs
		373741 RASN_HUMAN (1-189): 1.05e-80: 239: 100.00		ARAF_HUMAN (1-609): 0: 599: 99.34
+	Mm
		411149 RASK_MOUSE (1-189): 2.96e-85: 251: 87.90		ARAF_MOUSE (1-609): 0: 581: 94.25
+	Hs
		375142 RASK_HUMAN (16-189): 7.11e-85: 249: 85.71		BRAF_HUMAN (3-607): 0: 563: 55.88
+	Hs
		375069 RASK_HUMAN (16-189): 7.11e-85: 249: 85.71		RAF1_HUMAN (6-609): 0: 560: 59.80
+	Hs
		400574 RAB8A_HUMAN (4-185): 1.75e-84: 249: 36.02		RAF1_HUMAN (6-609): 0: 560: 59.80
+	Rn
		473535 RASK_RAT (1-189): 2.96e-85: 251: 87.90		RAF1_RAT (6-609): 0: 552: 59.31
+	Hs
		373754 RASN_HUMAN (1-189): 1.05e-80: 239: 100.00		BRAF_HUMAN (3-607): 0: 563: 55.88
+	Mm
		746565 RASK_MOUSE (1-189): 2.96e-85: 251: 87.90		RAF1_MOUSE (6-609): 0: 550: 59.15
+	Hs
		373722 RASN_HUMAN (1-189): 1.05e-80: 239: 100.00		RAF1_HUMAN (6-609): 0: 560: 59.80
+	Rn
		541167 RASH_RAT (16-188): 6.69e-79: 234: 84.39		RAF1_RAT (6-609): 0: 552: 59.31
+	Dm
		473590 RAS1_DROME (1-170): 2.88e-90: 263: 83.53		KRAF1_DROME (5-606): 1.94e-146: 442: 42.09
+	Ce
		653993 LET60_CAEEL (1-181): 1.4e-88: 259: 76.80		KRAF1_CAEEL (6-587): 3.18e-133: 410: 34.00
+	Hs
		375097 RASK_HUMAN (16-189): 7.11e-85: 249: 85.71		YES_HUMAN (292-571): 3.94e-104: 327: 31.34
+	Hs
		375131 RASK_HUMAN (16-189): 7.11e-85: 249: 85.71		SRC_HUMAN (285-571): 2.55e-104: 327: 30.24
+	Hs
		463098 RAB35_HUMAN (4-171): 6.54e-84: 247: 34.71		YES_HUMAN (292-571): 3.94e-104: 327: 31.34
+	Hs
		373734 RASN_HUMAN (1-189): 1.05e-80: 239: 100.00		YES_HUMAN (292-571): 3.94e-104: 327: 31.34
+	Hs
		373749 RASN_HUMAN (1-189): 1.05e-80: 239: 100.00		SRC_HUMAN (285-571): 2.55e-104: 327: 30.24
+	Mm
		746568 RASK_MOUSE (1-189): 2.96e-85: 251: 87.90		Q9JJU6_MOUSE (3-273): 2.63e-56: 196: 44.17