HitPredict

Interactor 1: A4 (P05067)
Amyloid-beta precursor protein (APP) (ABPP) (APPI) (Alzheimer disease amyloid A4 protein homolog) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Amyloid-beta (A4) precursor protein) (Amyloid-beta A4 protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99 (Beta-secretase C-terminal fragment) (Beta-CTF); Amyloid-beta protein 42 (Abeta42) (Beta-APP42); Amyloid-beta protein 40 (Abeta40) (Beta-APP40); C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF); P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)); C31]
Gene: APP

Interactor 2: KLK6 (Q92876)
Kallikrein-6 (EC 3.4.21.-) (Neurosin) (Protease M) (SP59) (Serine protease 18) (Serine protease 9) (Zyme)
Gene: KLK6

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.789

Property	Details
Experiments	12878203, 32814053
Identification Methods	protease assay, two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	protein cleavage, physical association

Property	Details
Interacting Pfam domains	Kunitz_BPTI~Trypsin
Common GO terms	GO:0005576 - extracellular region GO:0005615 - extracellular space GO:0005737 - cytoplasm GO:0005783 - endoplasmic reticulum GO:0007417 - central nervous system development
Homologous interactions	13

Homologous interactions

Color coding for hit scores

	1 770 Hs			1 244
+	Rn
		341604 A4_RAT (1-770): 0: 1021: 96.88		TRY2_RAT (2-243): 3.83e-120: 343: 42.92
+	Hs
		394096 A4_HUMAN (19-770): 0: 999: 100.00		KLK2_HUMAN (1-243): 9.04e-106: 308: 42.47
+	Hs
		394348 A4_HUMAN (19-770): 0: 999: 100.00		KLK7_HUMAN (13-242): 8.34e-100: 292: 40.52
+	Hs
		395867 A4_HUMAN (19-770): 0: 999: 100.00		TMPS4_HUMAN (5-244): 3.8e-97: 292: 32.68
+	Hs
		396025 A4_HUMAN (19-770): 0: 999: 100.00		KLK9_HUMAN (19-242): 1.45e-97: 286: 43.48
+	Hs
		589596 APLP2_HUMAN (3-769): 0: 858: 49.17		KLK2_HUMAN (1-243): 9.04e-106: 308: 42.47
+	Hs
		987549 APLP2_HUMAN (3-769): 0: 858: 49.17		KLK15_HUMAN (12-242): 4.73e-103: 300: 44.03
+	Hs
		371454 A4_HUMAN (19-770): 0: 999: 100.00		FA11_HUMAN (21-243): 1.78e-38: 142: 38.08
+	Hs
		498395 TFPI1_HUMAN (251-411): 3.44e-25: 108: 17.01		TRY3_HUMAN (17-243): 6.43e-112: 325: 42.36
+	Bt
		341601 BPT1_BOVIN (268-347): 2.01e-24: 100: 36.25		TRY1_BOVIN (2-243): 1.5e-115: 332: 42.92
+	Hs
		199968 SPIT2_HUMAN (282-399): 1.76e-24: 105: 18.83		KLK11_HUMAN (19-242): 2.29e-105: 307: 44.78
+	Hs
		199478 SPIT1_HUMAN (174-344): 2.57e-17: 88.1: 18.97		KLK15_HUMAN (12-242): 4.73e-103: 300: 44.03
+	Hs
		199896 SPIT2_HUMAN (282-399): 1.76e-24: 105: 18.83		PRSS8_HUMAN (12-241): 1.32e-94: 282: 34.13