HitPredict

Interactor 1: RAF1 (P04049)
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Proto-oncogene c-RAF) (cRaf) (Raf-1)
Gene: RAF1

Interactor 2: 1433G (P61981)
14-3-3 protein gamma (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein gamma, N-terminally processed]
Gene: YWHAG

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	1.00	0.984

Property	Details
Experiments	25852190, 31980649, 10620507, 32707033, 16041367, 17353931, 28205554, 10433554, 33961781, 35271311, 36931259, 16093354, 28514442, unassigned2173, 37045861, 11697890
Identification Methods	affinity chromatography technology, fluorescent resonance energy transfer, proximity labelling technology, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, pull down, biochemical
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	14-3-3~C1_1, 14-3-3~RBD
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 648 Hs			1 247
+	Hs
		400596 RAF1_HUMAN (1-648): 0: 637: 100.00		1433E_HUMAN (1-244): 2.03e-141: 398: 63.53
+	Mm
		967123 RAF1_MOUSE (1-648): 0: 632: 97.69		1433G_MOUSE (1-247): 3.17e-139: 392: 100.00
+	Hs
		400692 RAF1_HUMAN (1-648): 0: 637: 100.00		1433F_HUMAN (1-246): 2.51e-135: 382: 86.99
+	Hs
		400369 RAF1_HUMAN (1-648): 0: 637: 100.00		1433B_HUMAN (2-247): 1.92e-109: 317: 75.40
+	Hs
		400622 RAF1_HUMAN (1-648): 0: 637: 100.00		1433Z_HUMAN (2-245): 2.4e-108: 314: 74.59
+	Mm
		990045 RAF1_MOUSE (1-648): 0: 632: 97.69		1433Z_MOUSE (2-245): 8.5e-109: 315: 74.59
+	Hs
		400312 RAF1_HUMAN (1-648): 0: 637: 100.00		1433T_HUMAN (2-245): 5.55e-105: 305: 70.33
+	Hs
		400370 RAF1_HUMAN (1-648): 0: 637: 100.00		1433S_HUMAN (2-245): 1.87e-98: 289: 65.16
+	Hs
		116544 H7C560_HUMAN (39-647): 5.18e-166: 495: 56.02		1433E_HUMAN (1-244): 2.03e-141: 398: 63.53
+	Hs
		578439 BRAF_HUMAN (39-647): 9.6e-166: 494: 56.02		1433E_HUMAN (1-244): 2.03e-141: 398: 63.53
+	Dm
		541190 KRAF1_DROME (45-647): 8.65e-166: 494: 44.98		1433E_DROME (1-243): 1.59e-140: 396: 63.37
+	Hs
		578437 BRAF_HUMAN (39-647): 9.6e-166: 494: 56.02		1433G_HUMAN (1-247): 3.17e-139: 392: 100.00
+	Gg
		1050122 BRAF_CHICK (38-647): 1.36e-164: 491: 56.57		1433G_CHICK (1-247): 7.3e-140: 393: 99.60
+	Hs
		578462 BRAF_HUMAN (39-647): 9.6e-166: 494: 56.02		1433F_HUMAN (1-246): 2.51e-135: 382: 86.99
+	Hs
		578401 BRAF_HUMAN (39-647): 9.6e-166: 494: 56.02		1433B_HUMAN (2-247): 1.92e-109: 317: 75.40
+	Hs
		116545 H7C560_HUMAN (39-647): 5.18e-166: 495: 56.02		1433Z_HUMAN (2-245): 2.4e-108: 314: 74.59
+	Dm
		541183 KRAF1_DROME (45-647): 8.65e-166: 494: 44.98		1433Z_DROME (2-242): 1.37e-108: 314: 74.69
+	Hs
		578447 BRAF_HUMAN (39-647): 9.6e-166: 494: 56.02		1433Z_HUMAN (2-245): 2.4e-108: 314: 74.59
+	Hs
		578385 BRAF_HUMAN (39-647): 9.6e-166: 494: 56.02		1433T_HUMAN (2-245): 5.55e-105: 305: 70.33
+	Hs
		578402 BRAF_HUMAN (39-647): 9.6e-166: 494: 56.02		1433S_HUMAN (2-245): 1.87e-98: 289: 65.16