HitPredict

Interactor 1: SPD2B (A1X283)
SH3 and PX domain-containing protein 2B (Adapter protein HOFI) (Factor for adipocyte differentiation 49) (Tyrosine kinase substrate with four SH3 domains)
Gene: SH3PXD2B

Interactor 2: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	21706016
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1, SH2~SH3_2, SH3_1~SH3_2
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 911 Hs			1 217
+	Hs
		51054 SPD2B_HUMAN (1-911): 3.95e-155: 481: 100.00		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Mm
		1141129 VAV2_MOUSE (4-424): 1.09e-50: 194: 12.72		GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54
+	Hs
		855781 VAV2_HUMAN (4-424): 1.6e-46: 182: 11.94		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Hs
		557655 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Hs
		924630 SPD2A_HUMAN (202-910): 7.56e-41: 165: 17.96		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Hs
		557594 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Hs
		337337 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46
+	Hs
		557630 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04
+	Mm
		337637 NCK1_MOUSE (137-292): 1.46e-45: 170: 27.22		ABL1_MOUSE (4-139): 4.07e-22: 95.9: 35.46
+	Hs
		412098 VAV2_HUMAN (4-424): 1.6e-46: 182: 11.94		FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58
+	Hs
		1349275 ITSN2_HUMAN (40-605): 5.76e-43: 172: 12.11		ITSN2_HUMAN (2-61): 5.91e-15: 75: 36.67
+	Hs
		411986 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58
+	Mm
		763403 NCK1_MOUSE (137-292): 1.46e-45: 170: 27.22		FYN_MOUSE (161-215): 4.44e-14: 72.3: 31.58
+	Hs
		1078919 ITSN2_HUMAN (40-605): 5.76e-43: 172: 12.11		ITSN1_HUMAN (137-216): 1.36e-12: 68.1: 25.00
+	Hs
		523022 SPD2A_HUMAN (202-910): 7.56e-41: 165: 17.96		SRC_HUMAN (161-215): 3.76e-11: 63.8: 31.58
+	Hs
		208989 NCK2_HUMAN (123-313): 2.32e-32: 131: 24.88		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Mm
		403443 P3C2A_MOUSE (7-143): 9.06e-40: 162: 28.78		SRC_MOUSE (161-215): 3.76e-11: 63.8: 31.58
+	Hs
		1078828 ITSN1_HUMAN (4-604): 1.16e-31: 136: 10.64		ITSN1_HUMAN (137-216): 1.36e-12: 68.1: 25.00
+	Hs
		433781 ITSN1_HUMAN (4-604): 1.16e-31: 136: 10.64		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47