HitPredict

Interactor 1: BRAF (P15056)
Serine/threonine-protein kinase B-raf (EC 2.7.11.1) (Proto-oncogene B-Raf) (p94) (v-Raf murine sarcoma viral oncogene homolog B1)
Gene: BRAF

Interactor 2: 1433S (P31947)
14-3-3 protein sigma (Epithelial cell marker protein 1) (Stratifin)
Gene: SFN

Species: Homo sapiens

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.90	1.00	0.954

Property	Details
Experiments	15778465, 27034005, 27684187, 28205554, 31980649, unassigned2173, 36931259
Identification Methods	affinity chromatography technology, fluorescent resonance energy transfer, anti tag coimmunoprecipitation, cross-linking study, tandem affinity purification
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	14-3-3~C1_1, 14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 766 Hs			1 248
+	Hs
		542849 BRAF_HUMAN (1-766): 0: 727: 100.00		1433E_HUMAN (2-246): 4.41e-146: 410: 60.41
+	Hs
		110869 H7C560_HUMAN (1-763): 0: 721: 99.87		1433E_HUMAN (2-246): 4.41e-146: 410: 60.41
+	Gg
		982842 BRAF_CHICK (18-766): 0: 709: 95.08		1433E_CHICK (2-246): 4.41e-146: 410: 60.41
+	Hs
		542857 BRAF_HUMAN (1-766): 0: 727: 100.00		1433Z_HUMAN (1-243): 5.4e-136: 384: 70.37
+	Mm
		250867 BRAF_MOUSE (12-766): 0: 678: 91.94		1433S_MOUSE (1-248): 4.84e-154: 429: 97.58
+	Hs
		110870 H7C560_HUMAN (1-763): 0: 721: 99.87		1433Z_HUMAN (1-243): 5.4e-136: 384: 70.37
+	Mm
		655938 BRAF_MOUSE (12-766): 0: 678: 91.94		1433E_MOUSE (2-246): 4.41e-146: 410: 60.41
+	Hs
		542820 BRAF_HUMAN (1-766): 0: 727: 100.00		1433T_HUMAN (1-238): 9.98e-108: 312: 71.85
+	Hs
		542827 BRAF_HUMAN (1-766): 0: 727: 100.00		1433B_HUMAN (1-239): 3.41e-100: 293: 68.62
+	Gg
		982840 BRAF_CHICK (18-766): 0: 709: 95.08		1433T_CHICK (1-238): 1.18e-106: 310: 71.01
+	Hs
		542847 BRAF_HUMAN (1-766): 0: 727: 100.00		1433G_HUMAN (1-242): 1.86e-98: 289: 65.16
+	Hs
		542868 BRAF_HUMAN (1-766): 0: 727: 100.00		1433F_HUMAN (2-242): 2.78e-96: 283: 64.20
+	Gg
		982836 BRAF_CHICK (18-766): 0: 709: 95.08		1433Z_CHICK (1-239): 2.54e-102: 298: 71.13
+	Gg
		982838 BRAF_CHICK (18-766): 0: 709: 95.08		1433B_CHICK (1-239): 4.1e-100: 293: 69.04
+	Gg
		982833 BRAF_CHICK (18-766): 0: 709: 95.08		1433G_CHICK (1-242): 1.6e-98: 289: 65.16
+	Gg
		982837 BRAF_CHICK (18-766): 0: 709: 95.08		Q5ZKJ2_CHICK (2-242): 1.74e-96: 284: 64.20
+	Hs
		46556 A0A2R8Y8E0_HUMAN (1-763): 0: 699: 94.89		1433B_HUMAN (1-239): 3.41e-100: 293: 68.62
+	Hs
		46557 A0A2R8Y8E0_HUMAN (1-763): 0: 699: 94.89		1433G_HUMAN (1-242): 1.86e-98: 289: 65.16
+	Hs
		46558 A0A2R8Y8E0_HUMAN (1-763): 0: 699: 94.89		1433F_HUMAN (2-242): 2.78e-96: 283: 64.20
+	Mm
		655936 BRAF_MOUSE (12-766): 0: 678: 91.94		1433G_MOUSE (1-242): 1.86e-98: 289: 65.16