HitPredict

Interactor 1: SMUF2 (A2A5Z6)
E3 ubiquitin-protein ligase SMURF2 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase SMURF2) (SMAD ubiquitination regulatory factor 2) (SMAD-specific E3 ubiquitin-protein ligase 2)
Gene: Smurf2

Interactor 2: RAC1 (P63001)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (p21-Rac1)
Gene: RAC1

Species: Mus musculus

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.44	0.60	0.515

Property	Details
Experiments	19379695
Identification Methods	affinity chromatography technology, luminescence based mammalian interactome mapping
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 748 Mm			1 192
+	Hs
		962505 SMUF2_HUMAN (1-748): 0: 837: 99.20		RHOA_HUMAN (5-192): 1.33e-75: 226: 57.98
+	Hs
		962511 SMUF1_HUMAN (1-748): 0: 740: 70.05		RHOA_HUMAN (5-192): 1.33e-75: 226: 57.98
+	Rn
		92860 D3ZCF5_RAT (1-748): 0: 725: 70.31		RHOA_RAT (5-192): 5.27e-76: 227: 57.98
+	Hs
		131927 WWP2_HUMAN (122-748): 0: 678: 42.41		RAC1_HUMAN (1-192): 4.32e-85: 250: 100.00
+	Hs
		131712 WWP2_HUMAN (122-748): 0: 678: 42.41		RAC2_HUMAN (1-192): 2.74e-81: 241: 92.19
+	Hs
		132297 WWP2_HUMAN (122-748): 0: 678: 42.41		RHOJ_HUMAN (2-189): 1.39e-80: 240: 60.64
+	Hs
		131872 WWP2_HUMAN (122-748): 0: 678: 42.41		RAC3_HUMAN (1-191): 3.71e-78: 233: 93.19
+	Hs
		131876 WWP2_HUMAN (122-748): 0: 678: 42.41		CDC42_HUMAN (1-192): 5.32e-78: 232: 70.31
+	Hs
		131951 WWP2_HUMAN (122-748): 0: 678: 42.41		RHOG_HUMAN (1-192): 1.18e-77: 231: 71.35
+	Hs
		131886 WWP2_HUMAN (122-748): 0: 678: 42.41		RHOA_HUMAN (5-192): 1.33e-75: 226: 57.98
+	Hs
		131677 WWP2_HUMAN (122-748): 0: 678: 42.41		RHOC_HUMAN (5-192): 3.53e-73: 220: 57.45
+	Hs
		131717 WWP2_HUMAN (122-748): 0: 678: 42.41		RHOQ_HUMAN (4-190): 7.78e-73: 220: 63.16
+	Hs
		988487 HUWE1_HUMAN (178-747): 0: 584: 33.56		RAC1_HUMAN (1-192): 4.32e-85: 250: 100.00
+	Hs
		955331 HUWE1_HUMAN (178-747): 0: 584: 33.56		CDC42_HUMAN (1-192): 5.32e-78: 232: 70.31
+	Hs
		988513 HACE1_HUMAN (164-743): 1.24e-169: 513: 28.48		RAC1_HUMAN (1-192): 4.32e-85: 250: 100.00
+	Hs
		1400021 NED4L_HUMAN (5-416): 3.47e-38: 154: 16.27		RHOJ_HUMAN (2-189): 1.39e-80: 240: 60.64
+	Hs
		581231 ITCH_HUMAN (1-373): 6.62e-26: 116: 17.33		RAC2_HUMAN (1-192): 2.74e-81: 241: 92.19
+	Hs
		953617 ITCH_HUMAN (1-373): 6.62e-26: 116: 17.33		RAC3_HUMAN (1-191): 3.71e-78: 233: 93.19
+	Hs
		466410 ITCH_HUMAN (1-373): 6.62e-26: 116: 17.33		RHOC_HUMAN (5-192): 3.53e-73: 220: 57.45
+	Hs
		601404 ITCH_HUMAN (1-373): 6.62e-26: 116: 17.33		RHOQ_HUMAN (4-190): 7.78e-73: 220: 63.16