HitPredict

Interactor 1: SPB1 (P25582)
27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase (EC 2.1.1.167) (2'-O-ribose RNA methyltransferase SPB1) (AdoMet-dependent rRNA methyltransferase SPB1) (S-adenosyl-L-methionine-dependent methyltransferase) (Suppressor of PAB1 protein 1)
Gene: SPB1

Interactor 2: NOP15 (P53927)
Ribosome biogenesis protein 15 (Nucleolar protein 15)
Gene: NOP15

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.79	0.60	0.691

Property	Details
Experiments	11805826, 16429126, 17922018, 37968396
Identification Methods	tandem affinity purification, affinity chromatography technology
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000463 - maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0005634 - nucleus GO:0005730 - nucleolus GO:0030687 - preribosome, large subunit precursor
Homologous interactions	7

Homologous interactions

Color coding for hit scores

	1 841 Sc			1 220
+	Hs
		1222389 SPB1_HUMAN (1-829): 0: 599: 34.62		MK67I_HUMAN (60-220): 8.1e-40: 139: 28.73
+	Dm
		1363289 Q9VXK5_DROME (1-836): 2.02e-142: 442: 33.49		Q9VCY1_DROME (72-204): 1.41e-38: 138: 29.20
+	Hs
		516587 CMTR1_HUMAN (4-348): 4.48e-87: 296: 14.07		PABP1_HUMAN (82-207): 1.24e-25: 105: 15.87
+	Hs
		1077417 CMTR1_HUMAN (4-348): 4.48e-87: 296: 14.07		ELAV1_HUMAN (85-207): 6.15e-25: 101: 18.70
+	Sc
		866283 MDN1_YEAST (303-790): 6.99e-32: 137: 9.25		NOP15_YEAST (1-220): 1.29e-46: 155: 100.00
+	Hs
		1316903 MDN1_HUMAN (287-776): 6.36e-11: 68.9: 9.98		MK67I_HUMAN (60-220): 8.1e-40: 139: 28.73
+	Hs
		517817 NFH_HUMAN (302-816): 8.75e-09: 61.5: 10.49		MK67I_HUMAN (60-220): 8.1e-40: 139: 28.73