HitPredict

Interactor 1: SPB4 (P25808)
ATP-dependent rRNA helicase SPB4 (EC 3.6.4.13) (Suppressor of PAB1 protein 4)
Gene: SPB4

Interactor 2: BRX1 (Q08235)
Ribosome biogenesis protein BRX1
Gene: BRX1

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.78	0.60	0.686

Property	Details
Experiments	16429126, 30568249, 37968396, 21825077
Identification Methods	affinity chromatography technology, tandem affinity purification
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000027 - ribosomal large subunit assembly GO:0003723 - RNA binding GO:0005730 - nucleolus GO:0030687 - preribosome, large subunit precursor
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 606 Sc			1 291
+	Sc
		976876 HAS1_YEAST (6-467): 1.01e-156: 460: 30.98		BRX1_YEAST (1-291): 1.12e-99: 295: 100.00
+	Sc
		624185 DBP2_YEAST (2-402): 7.47e-126: 383: 25.43		BRX1_YEAST (1-291): 1.12e-99: 295: 100.00
+	Sc
		733917 HAS1_YEAST (6-467): 1.01e-156: 460: 30.98		RPF2_YEAST (6-282): 1.45e-61: 199: 16.84
+	Sc
		866605 HAS1_YEAST (6-467): 1.01e-156: 460: 30.98		IMP4_YEAST (5-261): 1.73e-61: 198: 17.76
+	Sc
		638716 SPB4_YEAST (1-606): 2.46e-149: 445: 100.00		RPF2_YEAST (6-282): 1.45e-61: 199: 16.84
+	Sc
		755323 HAS1_YEAST (6-467): 1.01e-156: 460: 30.98		RPF1_YEAST (4-252): 5.84e-52: 173: 14.40
+	Hs
		1343143 DDX18_HUMAN (6-467): 8.02e-159: 472: 29.61		SSF1_HUMAN (12-281): 5.67e-45: 160: 12.87
+	Sc
		638747 SPB4_YEAST (1-606): 2.46e-149: 445: 100.00		RPF1_YEAST (4-252): 5.84e-52: 173: 14.40
+	Sc
		754899 HAS1_YEAST (6-467): 1.01e-156: 460: 30.98		SSF1_YEAST (10-197): 9.05e-34: 129: 15.64
+	Sc
		976889 HAS1_YEAST (6-467): 1.01e-156: 460: 30.98		SSF2_YEAST (10-197): 8.32e-33: 127: 15.17
+	Sc
		638745 SPB4_YEAST (1-606): 2.46e-149: 445: 100.00		SSF1_YEAST (10-197): 9.05e-34: 129: 15.64
+	Sc
		638893 SPB4_YEAST (1-606): 2.46e-149: 445: 100.00		SSF2_YEAST (10-197): 8.32e-33: 127: 15.17
+	Sc
		415968 DED1_YEAST (3-402): 8.68e-139: 418: 25.55		SSF1_YEAST (10-197): 9.05e-34: 129: 15.64
+	Sc
		699739 DRS1_YEAST (21-392): 3.9e-47: 178: 31.75		BRX1_YEAST (1-291): 1.12e-99: 295: 100.00
+	Sc
		593625 DBP4_YEAST (21-467): 7e-51: 189: 31.36		RPF2_YEAST (6-282): 1.45e-61: 199: 16.84
+	Sc
		593679 DBP4_YEAST (21-467): 7e-51: 189: 31.36		IMP4_YEAST (5-261): 1.73e-61: 198: 17.76
+	Sc
		699670 DRS1_YEAST (21-392): 3.9e-47: 178: 31.75		RPF2_YEAST (6-282): 1.45e-61: 199: 16.84
+	Sc
		699680 DRS1_YEAST (21-392): 3.9e-47: 178: 31.75		RPF1_YEAST (4-252): 5.84e-52: 173: 14.40
+	Sc
		699679 DRS1_YEAST (21-392): 3.9e-47: 178: 31.75		SSF1_YEAST (10-197): 9.05e-34: 129: 15.64
+	Sc
		699748 DRS1_YEAST (21-392): 3.9e-47: 178: 31.75		SSF2_YEAST (10-197): 8.32e-33: 127: 15.17