HitPredict

Interactor 1: BTK (P35991)
Tyrosine-protein kinase BTK (EC 2.7.10.2) (Agammaglobulinemia tyrosine kinase) (ATK) (B-cell progenitor kinase) (BPK) (Bruton tyrosine kinase) (Kinase EMB)
Gene: BTK

Interactor 2: GRB2 (Q60631)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (SH2/SH3 adapter GRB2)
Gene: GRB2

Species: Mus musculus

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.34	1.00	0.590

Property	Details
Experiments	15102471
Identification Methods	two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	PH~SH3_1, PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1, SH2~SH2, SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0050853 - B cell receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 659 Mm			1 217
+	Hs
		924503 BTK_HUMAN (1-659): 0: 772: 98.33		GRB2_HUMAN (1-217): 6.92e-74: 224: 99.54
+	Hs
		788982 TEC_HUMAN (5-658): 0: 695: 53.73		GRB2_HUMAN (1-217): 6.92e-74: 224: 99.54
+	Hs
		924520 ITK_HUMAN (4-657): 0: 663: 49.39		GRB2_HUMAN (1-217): 6.92e-74: 224: 99.54
+	Hs
		337440 BTK_HUMAN (1-659): 0: 772: 98.33		ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46
+	Hs
		548690 BTK_HUMAN (1-659): 0: 772: 98.33		VAV_HUMAN (3-60): 1.14e-15: 76.9: 43.10
+	Hs
		412147 BTK_HUMAN (1-659): 0: 772: 98.33		FYN_HUMAN (161-215): 4.88e-13: 69.2: 31.58
+	Hs
		442813 BTK_HUMAN (1-659): 0: 772: 98.33		HCK_HUMAN (116-215): 1.24e-11: 64.9: 19.61
+	Hs
		788974 TEC_HUMAN (5-658): 0: 695: 53.73		FRK_HUMAN (3-202): 7.94e-39: 141: 28.84
+	Hs
		433717 BTK_HUMAN (1-659): 0: 772: 98.33		LYN_HUMAN (136-215): 4.12e-10: 60.7: 24.39
+	Hs
		69206 A9UF07_HUMAN (56-659): 1.62e-179: 557: 33.33		GRB2_HUMAN (1-217): 6.92e-74: 224: 99.54
+	Hs
		411650 BMX_HUMAN (5-658): 0: 642: 46.90		LCK_HUMAN (3-202): 7.66e-38: 138: 28.91
+	Hs
		548654 TEC_HUMAN (5-658): 0: 695: 53.73		VAV_HUMAN (3-60): 1.14e-15: 76.9: 43.10
+	Hs
		433631 TEC_HUMAN (5-658): 0: 695: 53.73		LYN_HUMAN (136-215): 4.12e-10: 60.7: 24.39
+	Hs
		412155 ITK_HUMAN (4-657): 0: 663: 49.39		FYN_HUMAN (161-215): 4.88e-13: 69.2: 31.58
+	Hs
		522941 ITK_HUMAN (4-657): 0: 663: 49.39		SRC_HUMAN (160-215): 1.74e-10: 61.9: 31.03
+	Hs
		522868 BMX_HUMAN (5-658): 0: 642: 46.90		SRC_HUMAN (160-215): 1.74e-10: 61.9: 31.03
+	Rn
		989767 SRC_RAT (151-653): 0: 526: 33.85		LYN_RAT (3-202): 1.89e-38: 140: 27.49
+	Rn
		693648 SRC_RAT (151-653): 0: 526: 33.85		CSK_RAT (2-139): 1.15e-23: 99.4: 35.57
+	Mm
		403406 SRC_MOUSE (151-653): 0: 527: 33.92		SRC_MOUSE (80-215): 1.5e-10: 61.9: 17.24
+	Gg
		337663 SRC_CHICK (161-653): 0: 526: 33.99		SRC_CHICK (160-215): 1.69e-10: 61.9: 31.03