HitPredict

Interactor 1: AFAD (P55196)
Afadin (ALL1-fused gene from chromosome 6 protein) (Protein AF-6) (Afadin adherens junction formation factor)
Gene: AFDN

Interactor 2: RIT1 (Q92963)
GTP-binding protein Rit1 (EC 3.6.5.2) (Ras-like protein expressed in many tissues) (Ras-like without CAAX protein 1)
Gene: RIT1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.43	1.00	0.656

Property	Details
Experiments	10545207
Identification Methods	two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	DIL~Ras, PDZ~Ras, RA~Ras
Common GO terms	GO:0005886 - plasma membrane GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1824 Hs			1 219
+	Hs
		876485 AFAD_HUMAN (1-1824): 0: 1279: 100.00		RIT2_HUMAN (8-219): 6.77e-91: 267: 67.93
+	Hs
		876348 AFAD_HUMAN (1-1824): 0: 1279: 100.00		RAP1A_HUMAN (20-185): 1.66e-56: 179: 50.30
+	Hs
		876344 AFAD_HUMAN (1-1824): 0: 1279: 100.00		RRAS2_HUMAN (21-201): 4.46e-52: 168: 52.75
+	Hs
		348594 AFAD_HUMAN (1-1824): 0: 1279: 100.00		RASN_HUMAN (21-184): 3.19e-48: 158: 52.44
+	Hs
		349224 AFAD_HUMAN (1-1824): 0: 1279: 100.00		RASH_HUMAN (21-186): 2.02e-48: 158: 51.81
+	Hs
		350005 AFAD_HUMAN (1-1824): 0: 1279: 100.00		RASK_HUMAN (21-195): 1.28e-47: 156: 50.29
+	Dm
		442960 Q8IPP3_DROME (1-1732): 0: 876: 31.05		RAP1_DROME (20-182): 4.19e-59: 185: 52.15
+	Dm
		442967 Q95TT5_DROME (1-1642): 0: 835: 32.06		RAP1_DROME (20-182): 4.19e-59: 185: 52.15
+	Hs
		1079976 MYO5B_HUMAN (102-939): 4.92e-118: 416: 14.72		RB11B_HUMAN (11-210): 2.93e-81: 243: 27.59
+	Hs
		894825 MYO5B_HUMAN (102-939): 4.92e-118: 416: 14.72		RAB8A_HUMAN (14-217): 8.93e-78: 233: 29.27
+	Hs
		914453 MYO5B_HUMAN (102-939): 4.92e-118: 416: 14.72		RB11A_HUMAN (11-218): 1.48e-77: 233: 26.64
+	Rn
		914521 MYO5B_RAT (102-939): 4.23e-117: 413: 14.69		RB11A_RAT (11-218): 1.48e-77: 233: 26.64
+	Dm
		442954 Q24279_DROME (639-1315): 3.98e-97: 351: 38.74		RAP1_DROME (20-182): 4.19e-59: 185: 52.15
+	Hs
		914346 KI13A_HUMAN (184-691): 2.35e-70: 264: 13.74		RB11A_HUMAN (11-218): 1.48e-77: 233: 26.64
+	Hs
		349388 RAIN_HUMAN (244-982): 5.22e-96: 336: 16.01		RASH_HUMAN (21-186): 2.02e-48: 158: 51.81
+	Hs
		914382 KI13B_HUMAN (184-691): 5.1e-66: 250: 12.64		RB11A_HUMAN (11-218): 1.48e-77: 233: 26.64
+	Hs
		320127 KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67		RAB3D_HUMAN (1-218): 2.66e-77: 233: 26.36
+	Hs
		348944 KI13B_HUMAN (184-691): 5.1e-66: 250: 12.64		RASN_HUMAN (21-184): 3.19e-48: 158: 52.44
+	Hs
		488072 KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67		RRAS_HUMAN (19-188): 8.04e-48: 158: 51.77
+	Hs
		506390 KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67		RALA_HUMAN (22-188): 8e-48: 157: 46.11