HitPredict

Interactor 1: ACTG (P63261)
Actin, cytoplasmic 2 (EC 3.6.4.-) (Gamma-actin) [Cleaved into: Actin, cytoplasmic 2, N-terminally processed]
Gene: ACTG1

Interactor 2: COF2 (Q9Y281)
Cofilin-2 (Cofilin, muscle isoform)
Gene: CFL2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.973

Property	Details
Experiments	32296183, 25416956, 16189514, 25910212, 35271311, 37071682, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, cross-linking study, pull down, split luciferase complementation, two hybrid bait and prey pooling approach, two hybrid array, validated two hybrid, anti tag coimmunoprecipitation, two hybrid pooling approach and 1 others more... two hybrid prey pooling approach
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	Actin~Cofilin_ADF
Common GO terms	GO:0005615 - extracellular space GO:0005737 - cytoplasm GO:0045214 - sarcomere organization GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 375 Hs			1 166
+	Hs
		618104 ACTBL_HUMAN (2-375): 0: 734: 91.44		COF1_HUMAN (1-166): 3.75e-73: 218: 80.72
+	Hs
		1122425 ACTBL_HUMAN (2-375): 0: 734: 91.44		TWF2_HUMAN (3-151): 2.43e-29: 111: 18.42
+	Hs
		1092828 Q1KLZ0_HUMAN (1-375): 0: 604: 98.93		Q549N0_HUMAN (1-166): 1.38e-73: 219: 100.00
+	Mm
		578583 ACTB_MOUSE (1-375): 0: 604: 98.93		COF1_MOUSE (1-166): 1.72e-73: 219: 80.72
+	Hs
		891817 ACTB_HUMAN (1-375): 0: 604: 98.93		Q549N0_HUMAN (1-166): 1.38e-73: 219: 100.00
+	Hs
		892124 ACTB_HUMAN (1-375): 0: 604: 98.93		COF2_HUMAN (1-166): 1.38e-73: 219: 100.00
+	Hs
		929891 ACTG_HUMAN (1-375): 0: 604: 100.00		Q549N0_HUMAN (1-166): 1.38e-73: 219: 100.00
+	Hs
		618038 ACTB_HUMAN (1-375): 0: 604: 98.93		COF1_HUMAN (1-166): 3.75e-73: 218: 80.72
+	Hs
		618049 ACTG_HUMAN (1-375): 0: 604: 100.00		COF1_HUMAN (1-166): 3.75e-73: 218: 80.72
+	Hs
		618096 Q1KLZ0_HUMAN (1-375): 0: 604: 98.93		COF1_HUMAN (1-166): 3.75e-73: 218: 80.72
+	Rn
		799735 ACTB_RAT (1-375): 0: 604: 98.93		COF1_RAT (1-166): 3.36e-73: 218: 81.33
+	Hs
		891649 ACTB_HUMAN (1-375): 0: 604: 98.93		DEST_HUMAN (1-166): 2.97e-69: 208: 68.68
+	Hs
		894652 ACTG_HUMAN (1-375): 0: 604: 100.00		DEST_HUMAN (1-166): 2.97e-69: 208: 68.68
+	Hs
		894665 Q1KLZ0_HUMAN (1-375): 0: 604: 98.93		DEST_HUMAN (1-166): 2.97e-69: 208: 68.68
+	Hs
		891728 ACTB_HUMAN (1-375): 0: 604: 98.93		TWF1_HUMAN (3-156): 3.59e-33: 121: 21.66
+	Hs
		929835 ACTG_HUMAN (1-375): 0: 604: 100.00		TWF1_HUMAN (3-156): 3.59e-33: 121: 21.66
+	Hs
		891650 ACTB_HUMAN (1-375): 0: 604: 98.93		GMFB_HUMAN (1-154): 2.3e-33: 116: 19.11
+	Hs
		894697 ACTG_HUMAN (1-375): 0: 604: 100.00		GMFB_HUMAN (1-154): 2.3e-33: 116: 19.11
+	Hs
		891834 ACTB_HUMAN (1-375): 0: 604: 98.93		TWF2_HUMAN (3-151): 2.43e-29: 111: 18.42
+	Hs
		929897 ACTG_HUMAN (1-375): 0: 604: 100.00		TWF2_HUMAN (3-151): 2.43e-29: 111: 18.42