HitPredict

Interactor 1: M4K4 (P97820)
Mitogen-activated protein kinase kinase kinase kinase 4 (EC 2.7.11.1) (HPK/GCK-like kinase HGK) (MAPK/ERK kinase kinase kinase 4) (MEK kinase kinase 4) (MEKKK 4) (Nck-interacting kinase)
Gene: Map4k4

Interactor 2: NCK1 (Q99M51)
SH2/SH3 adapter protein Nck1 (Cytoplasmic protein Nck1) (NCK adapter protein 1) (Nck-1)
Gene: NCK1

Species: Mus musculus

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.34	0.50	0.417

Property	Details
Experiments	15102471
Identification Methods	two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	-
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1233 Mm			1 377
+	Hs
		348050 M4K4_HUMAN (1-1233): 0: 1083: 89.00		NCK1_HUMAN (1-377): 9.82e-102: 307: 98.94
+	Hs
		221993 M4K4_HUMAN (1-1233): 0: 1083: 89.00		NCK2_HUMAN (1-376): 8.35e-95: 289: 68.50
+	Hs
		594048 MINK1_HUMAN (1-1233): 0: 1052: 58.61		NCK1_HUMAN (1-377): 9.82e-102: 307: 98.94
+	Hs
		594130 TNIK_HUMAN (1-1233): 0: 1049: 60.44		NCK1_HUMAN (1-377): 9.82e-102: 307: 98.94
+	Hs
		222144 MINK1_HUMAN (1-1233): 0: 1052: 58.61		NCK2_HUMAN (1-376): 8.35e-95: 289: 68.50
+	Hs
		222223 TNIK_HUMAN (1-1233): 0: 1049: 60.44		NCK2_HUMAN (1-376): 8.35e-95: 289: 68.50
+	Mm
		1013573 TNIK_MOUSE (1-1233): 0: 1071: 62.05		SRBS2_MOUSE (46-287): 3.33e-23: 103: 18.55
+	Hs
		348101 M4K4_HUMAN (1-1233): 0: 1083: 89.00		GRB2_HUMAN (3-62): 3.51e-17: 81.3: 28.33
+	Hs
		987773 MINK1_HUMAN (1-1233): 0: 1052: 58.61		GRB2_HUMAN (3-62): 3.51e-17: 81.3: 28.33
+	Hs
		593917 MARK3_HUMAN (14-672): 4.32e-124: 402: 15.36		NCK1_HUMAN (1-377): 9.82e-102: 307: 98.94
+	Hs
		222200 SIK2_HUMAN (23-795): 1.1e-115: 384: 16.62		NCK2_HUMAN (1-376): 8.35e-95: 289: 68.50
+	Dm
		1269951 Q7KV88_DROME (4-771): 3.91e-118: 396: 41.70		NCK_DROME (5-374): 2.54e-72: 236: 42.04
+	Ce
		1171296 MIG15_CAEEL (6-751): 1.32e-112: 380: 38.04		Q95PW9_CAEEL (1-376): 1.13e-74: 238: 38.19
+	Hs
		699848 SIK2_HUMAN (23-795): 1.1e-115: 384: 16.62		P85A_HUMAN (241-376): 5.72e-21: 96.7: 19.57
+	Dm
		1061424 Q7KV89_DROME (4-771): 1.21e-118: 397: 41.57		GRAP_DROME (1-61): 5.12e-17: 80.8: 31.15
+	Hs
		440524 STK4_HUMAN (4-479): 2e-117: 376: 33.00		FYN_HUMAN (5-130): 6.38e-22: 99.1: 21.09
+	Hs
		440528 AAPK1_HUMAN (10-558): 2.5e-116: 375: 17.48		FYN_HUMAN (5-130): 6.38e-22: 99.1: 21.09
+	Hs
		987804 MYO3B_HUMAN (13-329): 1.43e-110: 379: 46.95		GRB2_HUMAN (3-62): 3.51e-17: 81.3: 28.33
+	Hs
		290339 KS6A4_HUMAN (1-730): 5.52e-116: 381: 17.04		SH3K1_HUMAN (7-125): 7.88e-14: 74.7: 19.01
+	Hs
		463219 SIK2_HUMAN (23-795): 1.1e-115: 384: 16.62		YES_HUMAN (47-233): 2.33e-10: 63.8: 17.26