HitPredict

Interactor 1: O02053 (O02053)
BUB1 N-terminal domain-containing protein
Gene: san-1

Interactor 2: BUB3 (Q9XWH0)
Mitotic checkpoint protein bub-3 (Budding uninhibited by benzimidazole 3)
Gene: bub-3

Species: Caenorhabditis elegans

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.68	1.00	0.826

Property	Details
Experiments	19123269, 14704431
Identification Methods	two hybrid, two hybrid array, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Mad3_BUB1_I~WD40
Common GO terms	GO:0000776 - kinetochore GO:0005654 - nucleoplasm GO:0007094 - mitotic spindle assembly checkpoint signaling GO:0051726 - regulation of cell cycle
Homologous interactions	10

Homologous interactions

Color coding for hit scores

	1 390 Ce			1 343
+	At
		187357 BUBR1_ARATH (14-236): 2.81e-88: 273: 22.32		BUB31_ARATH (8-343): 2.65e-98: 296: 39.53
+	Hs
		211001 BUB1_HUMAN (59-287): 5.54e-84: 278: 19.40		RAE1L_HUMAN (2-343): 4.12e-85: 263: 27.89
+	Hs
		210957 BUB1_HUMAN (59-287): 5.54e-84: 278: 19.40		BUB3_HUMAN (10-343): 2.81e-81: 251: 40.72
+	Hs
		236912 BUB1B_HUMAN (19-243): 6.85e-74: 250: 19.48		RAE1L_HUMAN (2-343): 4.12e-85: 263: 27.89
+	Dm
		51404 A1Z6I7_DROME (21-284): 1.14e-69: 240: 18.28		Q9VAJ2_DROME (13-343): 5.31e-87: 266: 42.60
+	Hs
		211084 BUB1B_HUMAN (19-243): 6.85e-74: 250: 19.48		BUB3_HUMAN (10-343): 2.81e-81: 251: 40.72
+	Dm
		1361250 Q9VMS5_DROME (49-200): 9.48e-50: 181: 18.42		Q9VAJ2_DROME (13-343): 5.31e-87: 266: 42.60
+	Sp
		194057 MAD3_SCHPO (17-282): 3.2e-70: 224: 16.55		BUB3_SCHPO (24-343): 4.02e-70: 223: 33.13
+	Sc
		643044 MAD3_YEAST (20-233): 1.11e-58: 200: 21.78		BUB3_YEAST (16-315): 3.11e-77: 242: 28.27
+	Sc
		643042 BUB1_YEAST (46-225): 4.38e-45: 168: 18.85		BUB3_YEAST (16-315): 3.11e-77: 242: 28.27