HitPredict

Interactor 1: BIG2 (Q9Y6D5)
Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (Brefeldin A-inhibited GEP 2) (ADP-ribosylation factor guanine nucleotide-exchange factor 2)
Gene: ARFGEF2

Interactor 2: BIG1 (Q9Y6D6)
Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (Brefeldin A-inhibited GEP 1) (ADP-ribosylation factor guanine nucleotide-exchange factor 1) (p200 ARF guanine nucleotide exchange factor) (p200 ARF-GEP1)
Gene: ARFGEF1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.85 1.00 0.924

The method score is based on:

Property Details
Experiments 10716990, 35271311, 36652389, 19332778, 22084092
Identification Methods affinity chromatography technology, cross-linking study, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation
Interaction Type physical association, association

The annotation score is based on:

Property Details
Interacting Pfam domains BIG2_C~BIG2_C, DCB~Sec7-like_HUS, Sec7~Sec7, Sec7~Sec7-like_HDS, Sec7~Sec7-like_HUS, Sec7-like_HUS~Sec7-like_HUS
Common GO terms GO:0000139 - Golgi membrane
GO:0005085 - guanyl-nucleotide exchange factor activity
GO:0005802 - trans-Golgi network
GO:0005829 - cytosol
GO:0006887 - exocytosis
and 6 others

more...
Homologous interactions 9

Homologous interactions

Color coding for hit scores

1 1785
Hs

1 1849
+ Zm
+ Sp
+ Sc
+ At
+ Sc
+ Hs
+ Hs
+ Dm
+ Hs

Service provided by Combinatics, Japan

Last updated: 11Aug2025